Yoo, Dongwan
Education
MRC Postdoctoral Fellow, Vaccine and Infectious Disease Organization (VIDO), Canada
PhD, University of Ottawa, Ottawa, Canada
MSc, Seoul National University, Seoul, Korea
DVM, Seoul National University, Seoul, Korea
Previous Professional Positions
특임교수, Seoul National University, Seoul, Korea
Visiting Professor, Leiden University Medical Center, Leiden, The Netherlands
Associate Professor, Department of Pathobiology, University of Guelph, Canada
Assistant Professor, Department of Pathobiology, University of Guelph, Canada
MRC Post-doctoral Fellow, Veterinary Infectious Disease Organization, Canada
Research Interests
Viruses infect humans, animals, plants, insects, and microbes, and many of human illnesses are caused by viruses. Animal viruses can cross species boundaries to infect human populations, and viral infections of domestic animals can cause an enormous economic and societal impact. Virus replication relies on host cell machineries, and thus during infection continuous interaction occurs between virus and host. Virology is the study of the molecular and cellular aspects of such infections and aims to develop control measures againist infections of medical and veterinary importance. We use Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Porcine Edpidemic Diarrhea Coronavirus (PEDV) as a model to study cell-virus interactions and viral evasion strategies against host immune surveillance. We have developed a reverse genetics system and using this system can generate genetically modified mutant viruses. This tool enables us to study various pathogenic mechanisms and to investigate roles that individual viral proteins play during infection.
Biography
Biography- Dr. Yoo studied Veterinary Medicine at Seoul National University in Korea. He completed his MSc in microbiology at the same institution and moved to Canada for further studies for virology in the Faculty of Health Sciences of the University of Ottawa. Following post-doctoral studies at the Vaccines and Infectious Disease Organization, Dr. Yoo was appointed Assistant Professor at Ontario Veterinary College University of Guelph where he initiated his current research on Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) as an emerged pathogen.
- Dr. Yoo's research strengths lie in molecular virology, pathogenesis, and modern vaccinology, which forms the basis of his current research themes for two RNA viruses, an arterivirus PRRSV and a coronavirus PEDV.
Courses Taught
- Virology and Viral Pathogenesis (PATH433/ MCB433)
- Mechanisms Viral Pathogenesis (PATH519/ MCB561)
- Pathobiologic Basis of Disease II (VM607)
- Thesis Research (PATH599)
- Seminar/Journal Club (PATH590)
- Special Problems (PATH592)
- Undergraduate Research (PATH 290)
- Biochemistry Senior Thesis Research (BIOC492)
- Research Project (MCB290)
Selected Publications
Wu, X., Hu, Y., Sui, C., Pan, L., Yoo, D., Miller, L., Lee, C., Cong, X., Li, J., Du, Y., & Qi, J. (2022). Multiple Site SUMOylation of FMDV 3C Protease and Its Negative Role in Viral Replication. J. Virol. 96:e0061222.
Su, C-M., Wang, L., Yoo, D. (2021). Activation of NF-kB and induction of inflammatory cytokine expressions mediated by ORF7a protein of SARS-CoV-2. Sci. Reports 11:13464.
Sui, C., Jiang, D., Wu, X., Liu, S., Li, F., Pan, L., Cong, X., Li, J., Yoo, D., Rock, D., Miller, L., Lee, C., Qi, J., Du, Y. (2021). Inhibition of antiviral innate immunity by foot-and-mouth disease virus Lpro through interaction with N-terminal domain of swine RNase L. J. Virol. 95:e0036121.
Ke, H., Han, M., Kim, J., Kurt E. Gustin, Yoo, D. (2019). Porcine reproductive and respiratory syndrome virus nsp1-beta interaction with the nucleoporin 62 facilitates virus replication in vitro. J. Virol. 93:e00469-19 (Cover page article, Volume 93, Issue 14, July 15, 2019).
Hou, Y., Ke, H. Kim, J., Yoo, D., Su, Y., Boley, P., Saif, L. J., Wang, Q. 2019. Engineering an effective and safe live attenuated PEDV vaccine candidate via inactivation of the viral 2'-O methyltransferase and the endocytosis signal of the spike protein. J. Virol. 93:e00406-19.
Zhang, Q., Ke, H., Blikslager, A., Fujita, T., Yoo, D. 2018. Type III interferon restriction by porcine epidemic diarrhea virus and the role of viral protein nsp1 in IRF1 signaling. J. Virol. 92:e01677-17.
Han, M., Ke, H., Zhang, Q., Yoo, D. 2017.Nuclear imprisonment of host cellular mRNA by nsp1ß protein of porcine reproductive and respiratory syndrome virus. Virology 505: 42-55 (Cover page article, Volume 505, May 2017).
Du Y, Bi J, Liu J, Liu X, Wu X, Jiang P, Yoo D, Zhang Y, Wu J, Wan R, Zhao X, Guo L, Sun W, Cong X, Chen L, Wang J. 2014. 3Cpro of FMDV antagonizes IFN signaling pathway by blocking STAT1/STAT2 nuclear translocation. J. Virol. 88:4908-4920.
Du Y, Pattnaik AK, Song C, Yoo D, Li G. 2012. Glycosyl-phosphatidylinositol (GPI)-anchored membrane association of the porcine reproductive and respiratory syndrome virus GP4 glycoprotein and its co-localization with CD163 in lipid rafts. Virology 424:18-31.
Song C, Krell P, Yoo D. 2010. Nonstructural protein 1a subunit-based inhibition of NF-kB activation and suppression of interferon-ß production by porcine reproductive and respiratory syndrome virus. Virology 407:268-280.
Lee C, Hodgins DC, Calvert JG, Welch SK, Jolie R, Yoo D. 2006. Mutations in the nuclear localization signal of the porcine reproductive and respiratory syndrome virus nucleocapsid protein attenuate virus replication. Virology 346:238-250 (Cover page article, Volume 346 Issue 1).
Yoo D, Wootton S, Li G, Song C, Rowland RR. 2003. Co-localization and interaction of the porcine arterivirus nucleocapsid protein with the small nucleolar RNA-associated protein fibrillarin. J. Virol. 77:12173-12183.
Wootton S, Yoo D. 2003. Homo-oligomerization of the porcine reproductive and respiratory syndrome virus nucleocapsid protein and the role of disulfide linkages. J. Virol. 77: 4546-4557.
Wootton S, Rowland RR, Yoo D.2002. Phosphorylation of the porcine reproductive and respiratory syndrome virus nucleocapsid protein. J. Virol. 76:10569-10576.
Links
Current Projects
- Immune Modulation and Evasion Strategy of PRRS Virus
- PRRS Virus as a Multivalent Vaccine Vector
- Molecular Bases for Interferon Modulation by PRRS Virus and PEDV
Grants
- USDA NIFA 2018-2022 PRRSV regulation for inflammatory regulators (PI)
- USDA NIFA 2013-2018 Immune Evasion of PRRSV and Vaccine Design (PI)
- USDA CSREES-NRI 2008-2012, Immune Modulation of PRRSV (PI)
- USDA HATCH, PRRS Virus as a Multivalent Vaccine Vector (PI)
- NIH T32, Infection Biology Training Program (Co-PI)
- NIH T35, Summer Training Translational Biomedical Research (Co-PI)
Honors and Awards
- Gordon and Helen Kruger Research Excellence Award (2018)
- Pfizer Award for Research Excellence (2012)
- Presidential Distinguished Professor Award (Research) (2003)
- Pfizer Faculty Award for Research Excellence (2003)
- Honorary Senior Scientist, Korean Ministry of Agriculture, Rural Development Administration (1992-2003)
Professional Affiliations
- Editorial Board - Virology (Elsevier)
- Editorial Board - Virus Research (Elsevier)
- Editorial Board - Animal Health Research Review (Cambridge University Press)
- Associate Editor - Journal of Veterinary Science (Korean Society of Veternary Science)
- American Society for Virology (life-time member)
- American Society for Microbiology (member)
- Conference of Research Workers in Animal Diseases (member)
Service Activities
- 18th International Nidovirus Symposium 2014, Scientific Program Committee, Spain
- International Committee on Taxonomy of Viruses, Arterivirus Study Group 2005-2011
- International Pig Veterinary Society Conference 2012 Scientific Committee, Korea
- International Pig Veterinary Society Conference 2010 Scientific Committee, Canada
- 17th International Nidovirus Symposium 2011, Scientific Program Committee, Michigan