Affiliate, Department of Biochemistry, Division of Nutritional Science, Neuroscience Program, Department of Bioengineering & Beckman Institute
Affiliate, Cancer Center of Illinois
- PostDoc, University of Illinois, Urbana Champaign
- PhD, Princeton University
- MSc, Indian Institute of Technology, Kanpur, India
- BSc, Stephen's College, Delhi University, India
Metabolism of Omega-3 fatty acids and Endocannabinoids by CYP epoxygenases to yield Anti-Inflammatory Lipid Metabolites
Our laboratory focuses on understanding how dietary fatty acids gets converted to anti-inflammatory lipid signaling molecules by epoxygenases. Recently, we have been investigating about biotransformation of both endocannabinoids and phytocannabinoids. Research in our laboratory is highly interdisciplinary and collaborative.
Please visit Das Laboratory website
VM 602 (Biochemistry)
VM603 (GI Physiology)
CB 449/FSHN 480 Basic Toxicology
2017. NIH R01 Grant from NIGMS
2016. Platform Speaker, Microsomal and Drug Oxidations
2015. American Heart Association, National Scientist Development Grant
2015. Lightening Talk LIPID MAPS meeting
2014. Lightening Talk LIPID MAPS meetig
2014. NIEHS funded travel award for International Winter Eicosanoid Conference
2013. Moog Lecture Series, Hauptman-Woodward Medical Research Institute
2013. ACS Workshop Travel Award
2012. Pew Scholar Nominee, UIUC campus
2010. ACS Biological Division Travel Award
2007. Outstanding Researcher Award, NSF-NSEC.
2006-11. NSF-(NSEC) Postdoctoral Fellowship.
2005. FMC Corporation Graduate Fellowship, Princeton.
2004. Dean’s List Finalist for Honorific Fellowship, Princeton.
2001. Science and Engineering First Year Princeton Graduate Fellowship.
1998. CSIR-JRF Fellowship, India.
1993-96. Merit List Scholarship, Delhi University, India.
Awards to Students
2015, Albert Doby award to Daniel McDougle
2015, Fulbright Scholarship to Ms. Susan Zelasko
2015, American Heart Association Fellowship to John Krapf
2015, Highest Distinction Awarded to Mr. Amogh Kambalyal and Ms. Susan Zelasko
2014, Best poster award to Ms. Susan Zelasko
2014, American Heart Association Fellowship to Daniel McDougle
2014, American Heart Association Fellowship to John Rouck
2014, William and Lycman Biochemistry Award to Amogh Kambalyal
2013, American Heart Association Fellowship to Susan Zelasko
2013. High Distinction for Undergraduate Thesis - Christopher Mularcyzk
2013. MCB Summer Fellowship - Susan Zelasko
Mechanisms of Eicosanoid Synthesizing Enzymes
Metabolism of Omega-3 and Omega-6 fatty acids by epoxygenases in the heart
Dietary consumption of omega-3 and omega-6 fatty acids have been linked to cardiovascular health benefits in humans. The central hypothesis is that the cardiovascular physiological effects of omega-3 and omega-6 fatty acids are partly mediated by the synthesis of eicosanoids via the epoxygenase (EPOX) pathway and also via reduction of platelet aggregation. The goal is to provide support to this central hypothesis through biochemical and physiological studies.
Novel Endocannabinoid Epoxide Metabolites
Omega-3 and omega-6 endocannabinoids ellicit similar response to Cannabis in the body. Our lab is studying the metabolism of these endocannabinoids through the epoxygenase pathway.
* denotes corresponding author or first author
37. Maroutsos, D., Huff, H., and Das, A*, "N-terminal modification to increase expression of Eukaryotic P450s in E.Coli - CYP5alpha Hydroxylase and CYP2J2 epoxygenase" Methods in Molecular Biology, Book Chapter. Submitted
36. McDougle, D.R., Abdeen,A., Adili, R., Caputo, M., Krapf, J., Johnson, R., Kilian, K., Holinstat, M., Das, A*, "Anti-inflammatory Omega-3 Endocannabinoid Epoxides", PNAS (Direct Submission), Accepted with Minor Revisions
35. Roy, J. Pondenis, H., Fan T.*, Das, A*, "Comparative Proteomic Analysis of Metastatic and Non-metastatic Osteosarcoma of Human and Canine Origin" PLOS One, Accepted with Minor Revisions
32. Rouck*, J.E., Bradley*, WB.,Kambalyal, A.,Parayil, A*. and Das, A*., "Expression and Purification of cytochrome P450 taxadiene 5alpha-hydroxylase in E.Coli", Protein Expression and Purification, Protein Expr Purif. 2017 Jan 18;132:60-67. doi: 10.1016/j.pep.2017.01.008.
33. Arnold, W., Baylon, J, Tajkorshid, E.* and Das, A.*,"Docosahexaenoic acid (DHA) preferentially binds to cardiovascular CYP2J2 and inhibits arachidonic acid (AA) metabolism" Biochemistry. 2016, 55(50):6969-6980
32. Biochemistry, 2015, 54 (41), pp 6299–6302. Plucinski, L., Gartia, M., Arnold, W., Ameen, A., Chang, T., Hsiao, A., Liu, G.*, Das, A.*, "Substrate Binding to CYP2J2- Nanodiscs Detected by Nanoplasmonic Lygurcus Cup Arrays" Biosens Bioelectron., 2016, 337-46.
31. Lim, SJ, McDougle, D., Das, A* and Smith, A.*, "Lipoprotein Nanoplatelets: Fluorescent, Zwitterionic Probes for Molecular and Cellular Imaging." Journal of the American Chemical Society, 2016 ASAP.
30. Roy, J. Pondenis, H., Fan T.*, Das, A*, "Direct Capture of Functional Proteins from Mammalian Plasma Membranes into Nanodiscs" Biochemistry, 2015, 6299–6302.
29. McDougle, D*., Baylon, J.* Meling D., Kambalyal, A., Tajkorshid, E.*, and Das, A* "Incorporation of Charged Residues in the CYP2J2 F-G Loop Disrupts CYP2J2-Lipid Bilayer Interactions" Biochimica et Biophysica Acta -Biomembranes, 2015, 2060-2470.
28. Zelasko, S, Meling, D. and Das A*. "A critical residue that controls the isomerization of prostaglandin in thromboxane synthase", Biophysical Chemistry, 2015, 34-40.
27. Wilcox, K., Das, A., Velasco, P., Sligar S.G. and Klein, W. "Nanoscale synaptic membrane mimetic allows unbiased high throughput screen that targets binding sites for Alzheimer’s-associated Aβ oligomers" 2015, PLOS ONE In Press
26. Zelasko, S., Arnold, W., Das, A* " Endocannabinoid Metabolism by Cytochrome P450 Monooxygenases.", Prostaglandins and Other Lipid Mediators. 2015, http://www.sciencedirect.com/science/article/pii/S1098882314000744
(News: Most downloaded article is at POLM)
25. McDougle, D., Kambalayal, A., Meling, D. and Das, A*. "Endocannabinoids - Anandamide and 2- Arachidonoylglycerol are Substrates for Human CYP2J2 Epoxygenase" Journal of Pharmacology and Experimental Therapeutics, 2014, 616-27.
23. Orlando, B., McDougle, D., Lucido, M., Eng, E., Stokes, D., Das, A*., and Malkowski, M*. "Cyclooxygenase-2 Catalysis and Inhibition in Lipid Bilayer Nanodiscs" Archives of Biochemistry and Biophysics 2014, 546, 33-40.
22. Srivarma, S., Mularcyzk, C. Meling, D. and Das, A*. "Substrate binding thermodynamics and kinetics to thromboxane synthase in model lipid bilayers", ChemBioChem. 2014, 15, 892
21. Zelasko S., Palaria, A., and Das, A* "Modifications to produce high-level expression of mammalian cytochrome P450s in recombinant expression systems" Protein Expression and Purification,2013,92,77-87
20. McDougle, D*., Palaria, A.*Magnetta E., Meling, D. and Das, A*. "Functional Studies of N-terminally modified CYP2J2 epoxygenase in Model Lipid Bilayers", Protein Science, 2013, 22,964 (Amrita Palaria and Daniel McDougle are equal authors)
19. Marty, M., Das, A., Sligar, S.G. “MALDI Mass Spectrometry of Membrane Proteins in Nanodiscs”, Analytical Bioanalytical Chemistry, 2012, Accepted.
(In News) Editor Highlight in Protein Science
15. Das. A., Zhao, J. Van Duyne, R. and Sligar, S.G. “Screening of Type I and II Drug Binding to Human Cytochrome P450-3A4 in Nanodiscs by Localized Surface Plasmon Resonance Spectroscopy”,Anal. Chem., 2009, 81 (10), 3754–3759.
11. Das. A., Grinkova, Y. and Sligar, S.G. “Redox Potential Control by Drug Binding to Cytochrome P450 3A4.” J. Am. Chem. Soc. 2007, 129 (45), 13778-13779
8. Zhao*, J., Das, A.*, Zhang, X, Schatz, G., Sligar, S., Van Duyne, R. “Resonance Surface Plasmon Spectroscopy: Low Molecular Weight Substrate Binding to Cytochrome P450” J. Am. Chem. Soc. 2006, 128 (34), 11004-11005. (* Co-first authors)
4. Das, A. “Electrochemical and Functional Studies of De Novo Alpha Helical Protein from a Designed Combinatorial Library.” Ph.D Thesis Princeton University November 2005
3. Das, A. Model Chemistry of Aerobic Acetylene Hydratase Enzymes. M.Sc. Thesis: I.I.T (Kanpur) 1998
• Van Duyne, R., Zhao, J., Das, A., Zhang, X., Schatz, G. and Sligar, S. (2006) “Resonance Surface Plasmon Spectroscopy: Low Molecular Weight Substrate Binding to Cytochrome P450” Patent Applied
• Klein, W., Sligar, S. G., Wilcox, K. Das, A. Velasco P., Marty, M. (2011) “An isolated set of central nervous system proteins that bind oligomers of amyloid beta (also known as ADDLs)” Invention Disclosure
NIH R01 Grant NIGMS
American Heart Association National SDG (Role: PI)
AHA - Grant Numbers- 15SDG25760064, 15UFEL25080175, 14PRE20130015, 14UFEL19990011, 13UFEL16890010
Research Board (Role: PI)
Manus Biosynthesis Industrial Funding (Role: PI)