Professional Profile

• PhD, Microbiology, University of New Mexico, Albuquerque
• MA, Microbiology, San Francisco State University

• Professor, Department of Diagnostic Medicine/ Pathobiology, Kansas State University
• Associate Professor, Department of Diagnostic Medicine/ Pathobiology, Kansas State University
• Associate Professor, Department of Biology-Microbiology, South Dakota State University
• Assistant Professor, Department of Biology-Microbiology, South Dakota State University
• Postdoctoral Fellow, Department of Microbiology, University of Minnesota
• Postdoctoral Fellow, Department of Pharmacology, University of Minnesota

• Emerging viral diseases of swine
• Swine immunity
• Novel detection and surveillance methods for detecting swine diseases
• Role of host genetics and genetic modification for the control of swine diseases

View Dr. Rowland's publications in PubMed.

Research interests in the Rowland lab center on addressing fundamental problems in the detection and control of infectious pig diseases caused by emerging and foreign animal diseases, including porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus (PCV), coronaviruses, and African swine fever virus (ASFV). Since 2008, the Rowland lab has been actively involved in understanding the role of host genetics in the response of pigs to infection, along with the application of genetic modification techniques for conferring disease resistance. A spinoff of the genetics work is the first discovery of pigs possessing a naturally occurring severe combined immunodeficiency (SCID). Rowland is actively involved in the training of graduate, undergraduate and DVM students. He has authored/coauthored more than 150 refereed publications in animal health.

• Petrovan, V, MV Murgia, P Wu, AD Lowe, W Jia, RRR Rowland. 2020. Epitope mapping of African swine fever virus (ASFV) structural protein, p54. Virus Res. In press.
  
  
• Stoian, A, RRR Rowland, V Petrovan, M Sheahan, MS Samuel, KM Whitworth, KD Wells, J Zhang, B Beaton, M Cigan, RS Prather. 2019. The use of cells from ANPEP knockout pigs to evaluate the role of aminopeptidase N (APN) as a receptor for porcine deltacoronavirus (PDCoV). Virology 541: 136-140.
  
  
• Popescu LN, Panyasing Y, Giménez-Lirola L, Zimmerman J, Rowland RRR. 2019. E2 and Erns isotype-specific antibody responses in serum and oral fluid after infection with classical swine fever virus (CSFV). Vet Microbiol. 235:265-269.
  
  
• Petrovan, V, F Yuan, Y Li, P Shang, MW Murgia, S Misra, RRR Rowland, Y Fang. 2020. Development and characterization of monoclonal antibodies against p30 protein of African swine fever virus. 2019. Virus Res. Epub 2020, 279: 197871.
  
  
• Whitworth, KM, RRR Rowland, V Petrovan, M Sheahan, AG Cino-Ozuna, Y Fang, R Hesse, A Mileham, MS Samuel, KD Wells, RS Prather. 2018. Resistance to coronavirus infection in amino peptidase N-deficient pigs. Transgenic Res. 2019; 28(1): 21–32.
  
  
• Heimerman, M, MV Murgia, P Wu, AD Lowe, W Jia, RRR Rowland. 2018. Linear epitopes in African swine fever virus (ASFV) p72 recognized by monoclonal antibodies prepared against baculovirus expressed antigen. J Vet Diagn Invest. 30:406-412.
  
  
• Prather, RS, KD Wells, KM Whitworth, MA Kerrigan, MS Samuel, A Mileham, LN Popescu, RRR Rowland. 2017. Knockout of maternal CD163 protects fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV). Sci Rep. 7:13371.
  
  
• Jaing, C, RRR Rowland, JE Allen, A Certoma, JB Thissen, J Bingham, B Rowe, JR White, J Wynne, D Johnson, N Gaudreault, DT Williams. 2017. Gene expression analysis of whole blood RNA from pigs infected with low and high pathogenic African swine fever viruses. Sci Rep. 7:10115.
  
  
• Popescu, LN, BR Trible, N Chen, RRR Rowland. 2017. GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) as a target for homologous and broadly neutralizing antibodies. Vet Microbiol. 209:90-96.
  
  
• Wells, KD, R Bardot, K M Whitworth, BR Trible, Y Fang, A Mileham, MA Kerrigan, MS Samuel, RS Prather, RRR Rowland. 2017. Substitution of porcine CD163 SRCR domain 5 with a CD163-like homolog confers resistance of pigs to genotype 1 but not genotype 2 porcine reproductive and respiratory syndrome (PRRS) viruses. J Virol. 91: EO1521-16, 1-11.
  
  
• Popescu, L, NN Gaudreault, K Whitworth, M Murgia, JC Nietfeld, A Mileham, M Samuel, KD Wells, RS Prather, RR Rowland. 2017. Genetically edited pigs lacking CD163 show no resistance following infection with the African swine fever virus isolate, Georgia 2007/1. Virology. 501:102-106.
  
  
• Niederwerder, MC, CJ Jaing, JB Thissen, AG Cino-Ozuna, KS McLoughlin, RR Rowland. 2016. Microbiome associations in pigs with the best and worst clinical outcomes following co-infection with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2). Vet Microbiol. 188:1-11.
  
  
• Schroyen, M, C Eisley, JE Koltes, E Fritz-Waters, I Choi, GS Plastow, L Guan, P Stothard, H Bao, A Kommadath, JM Reecy, JK Lunney, RR Rowland, JC Dekkers, CK Tuggle. 2016. Bioinformatic analyses in early host response to Porcine Reproductive and Respiratory Syndrome virus (PRRSV) reveals pathway differences between pigs with alternate genotypes for a major host response QTL. BMC Genomics. 17:196.
  
  
• Whitworth, KM, RRR Rowland, CL Ewen, BR Trible, MA Kerrigan, AG Cino-Ozuna, MS Samuel, D McLaren, A Mileham, KD Wells, RS Prather. 2016. Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus. Nature Biotech. 34:20-22.
  
  
• Niederwerder, MC, B Bhupinder , NVL Serão, BR Trible, MA Kerrigan, JK Lunney, JCM Dekkers, RRR Rowland.2015. Vaccination with a porcine reproductive and respiratory syndrome (PRRS) modified live virus vaccine followed by challenge with PRRSV and porcine circovirus type 2 (PCV2) protects against PRRS but enhances PCV2 replication and pathogenesis when compared to non-vaccinated co-challenged controls. J Clin Vaccine Immunol. 22:1244-1254.
  
  
• Trible, BR, LN Popescu, N Monday, J G Calvert, RRR Rowland. 2015. A single amino acid deletion in the matrix protein of porcine reproductive and respiratory syndrome virus confers resistance to a polyclonal swine antibody with broadly neutralizing activity. J Virol 89:6515-6520.
  
  
• Jaing, CJ, JB Thissen, SN Gardner, KS McLoughlin, P Hullinger, PJ, N Monday, MC Niederwerder, RR Rowland. 2015. Application of a pathogen microarray for the analysis of viruses and bacteria in clinical diagnostic samples from pigs. J Vet Diagn Invest. 27:313-325.
  
  
• Boddicker, NJ, A Bjorkquist, RR Rowland, JK Lunney, JM Reecy, JC Dekkers. 2014. Genome-wide association and genomic prediction for host response to porcine reproductive and respiratory syndrome virus infection. Genet Sel Evol. 46:18.
  
  
• Cino-Ozuna, AG, RRR Rowland, JC Nietfeld, MA Kerrigan, JC Dekkers, CR Wyatt. 2013. Preliminary findings of a previously unrecognized porcine primary immunodeficiency disorder. J Vet Pathol 50:144-146.
  
  
• Trible, BR, A Suddith, M Kerrigan, A Cino-Ozuna, R Hesse, RRR Rowland. 2012. Recognition of the different structural forms of the capsid protein (CP) determines the outcome following infection with porcine circovirus type 2 (PCV2). J Virol. 86:13508-13514.
  
  
• Lu, Z, J Zhang, CM Huang, YY Go, KS Faaberg, RR Rowland, PJ Timoney, UB Balasuriya. 2012. Chimeric viruses containing the N-terminal ectodomains of GP5 and M proteins of porcine reproductive and respiratory syndrome virus do not change the cellular tropism of equine arteritis virus. Virology. 432:99-109.
  
  
• Trible, BR, A Ramirez, A Suddith, A Fuller, M Kerrigan, R Hesse, J Nietfeld, B Guo, E Thacker, RRR Rowland. 2012. Antibody responses following vaccination versus infection in a porcine circovirus-type 2 (PCV2) disease model show distinct differences in virus neutralization and epitope recognition. Vaccine 30:4079-4085.
  
  
• Boddicker, N, EH Waide, RRR Rowland, JK Lunney, DJ Garrick1, JM Reecy, JCM Dekkers. 2012. Evidence for a major QTL associated with host response to porcine reproductive and respiratory syndrome virus challenge. J Anim Sci. 90:1733-1746.
• Trible, BR, M Kerrigan, N Crossland, M Potter, K Faaberg, R Hesse, RRR Rowland. 2011. Antibody recognition of porcine circovirus type 2 capsid protein epitopes associated with vaccination, infection and disease. Clin Vacc Immunol 18: 749-757.