NA PRRS Symposium

1:00 – 6:00 p.m. – Symposium Check-In/Onsite Registration

4:00 – 5:00 p.m. – NAPRRS/NC229 Organizing Committee Meeting

7:30 a.m. – 6:00 p.m. – Symposium Check-In/Onsite Registration

8:00 a.m. – 12:00 p.m. – Poster Set-Up

8:00 – 8:10 a.m. – Opening Remarks
Ying Fang and Raymond (Bob) Rowland

8:10 – 9:10 a.m. – Keynote Presentation: Wings of Worry: The Avian Flu Pandemic Potential
Daniel Perez, University of Georgia, USA

9:10 – 10:00 a.m. – Session 1: From Entry to Viral Infection
Moderators: Joan Lunney and Dongwan Yoo

• Role of N-glycosylation in PRRSV Infection
  Alberto Brandariz Nuez, University of Illinois, USA
• The Dimerization of PRRSV Nsp1alpha is Important for Viral Subgenomic RNA Synthesis
  Yanhua Li, Yangzhou University, China
• Lightning Talks
  • Cross-Species Analysis of CD163 Orthologs Reveals Key Domain for PRRSV Susceptibility
    Kassandra Durazo-Martinez, University of Nebraska-Lincoln, USA
  • An Intra-Family Conserved High-Order RNA Structure Within the M ORF is Important for PRRSV Subgenomic RNA Accumulation and Infectious Virus Production
    Chi Chen, University of Illinois, USA
  • A Novel Reverse Genetics System for PRRSV and the Generation of a Transcription Network-Reprogrammed Virus
    Junyu Tang, University of Illinois, USA
  • Segment-Specific Enteroids from Pig Small Intestine in Matrigel and Transwell Inserts: Susceptibility to Porcine Epidemic Diarrhea Virus (PEDV)
    Lu Yen, Iowa State University, USA

10:00 – 10:30 a.m. – Break

10:30 a.m. – 12:00 p.m. – Session 2: Insight into Viral Pathogenesis and Host Response
Moderators: Xiuqing Wang and Federico Zuckermann

• A Plausible Mechanism Responsible for the Variation of PRRS Virus Virulence
  Federico Zuckermann, University of Illinois, USA
• Defining Interferon Antagonism Hierarchy of Porcine Epidemic Diarrhea Virus
  Xufang Deng, Oklahoma State University, USA
• CRISPR-Cas9 Mediated Identification of Host Factors for Influenza Infection and Persistence
  Xiuqing Wang, South Dakota State University, USA
  
• Understanding Host X Pathogen Associations In Swine Infectious Diseases
  Daniel Ciobanu, University of Nebraska-Lincoln, USA
  
• Lightning Talks
  • scRNAseq Analysis of PBMCs During Acute PRRSV Infection: Searching for Host Cellular Markers to Predict Persistent Infection
    Mehak Kapoor, Iowa State University, USA
  • Single-Cell T cell Receptor Profiling in pig Lungs Using Single-Cell TCR Sequencing Analysis
    Yuhan Wen, University of Missouri, USA
  • Evaluation of PCV3 Humoral Responses in Experimentally Infected Pigs and Dynamic of Maternally Derived Antibodies in Piglets from Naturally Infected Sows
    Molly Kroeger, Iowa State University, USA
  • Investigation of Fetal Liver, Heart, and Thymus Transcriptomes for Prediction of Reproductive Failure
    Kristen Walker, USDA ARS, USA
  • Investigating MAIT Cell Contributions to Anti-Influenza Virus Immunity Using MR1 Knockout Pigs
    Laurie Touchard, University of Missouri, USA
  • Computationally Predicted T-Cell Epitope Trends for 30 Years of Wild-Type PRRSV-2 Strains from the USA
    Julia Baker, University of Minnesota, USA

12:00 – 2:00 p.m. – Session 3: Zoetis Lunch Session: Applied Research in the Swine Industry – Swine Disease Control and Elimination Update
Moderators: Jose Angulo and Jay Calvert

• 12:00 – 12:30 p.m.: Food Service
• Welcome and Introduction
  Jose Angulo, Zoetis, USA
• Novel Approaches to Swine Pathogen Monitoring and Surveillance: The Cornerstone for Effective
  Giovani Trevisan, Iowa State University, USA 
• The Art of Controlling and Eliminating Swine Diseases in the Field Using Science-Based Methods
  Bill Hollis, Carthage Veterinary Services, USA
• Q & A Session

2:00 – 3:30 p.m. – Session 4: Novel Vaccines and Vaccination Strategies
Moderators: Shafiqul Chowdhury and Elisa Crisci

• Efficacy of an Intranasal Naturally Attenuated Live PRRSV-2 Vaccine Against a Highly Virulent PRRSV-1 Strain
  Marti Cortey, Universitat Autònoma de Barcelona, Spain
• Novel Pseudorabies Virus (PRV) Vectored Subunit Vaccine Against African Swine Fever
  Shafiqul Chowdhury, Louisiana State University, USA
• Lipid Nanoparticle-Encapsulated DNA Vaccines: A Versatile Platform for Rapid Development of Vaccines Against Influenza A Viruses of Swine
  Hiep Vu, University of Nebraska-Lincoln, USA
• PRRSV-2 Immune Biobank for Vaccine Efficacy Prediction
  Elisa Crisci, North Carolina State University, USA
• Lightning Talks
  • Experimental Evidence of Vaccine-Driven Evolution of PRRSV-2 in Pigs-To-Pig Infection Chains
    Nakarin Pamornchainavakul, University of Minnesota, USA
  • Rational Design and Immunogenicity Evaluation of mRNA-Based Vaccine for African Swine Fever Virus
    Fangfeng Yuan, MIT, USA
  • Lipid Nanoparticle-Encapsulated DNA Vaccine Encoding African Swine Fever Virus p54 Antigen Elicits Robust Immune Responses in Pigs
    The Nguyen, University of Nebraksa-Lincoln, USA
  • Assessment of Homologous and Heterologous PCV2 Vaccine Efficacy in a PCV2d/PRRSV Co-Challenge Model
    Molly Kroeger, Iowa State University, USA

3:30 – 4:00 p.m. – Break

4:00 – 5:30 p.m. – NC229 Special Session
Moderator: Pablo Pineyro-Pineiro and Andreia Arruda

• NC229 Session Opening Remarks
  Pablo Pineyro, Iowa State University, USA
• NC229 Multi-State Program Summary and Future Perspective
  Hiep Vu, University of Nebraska, USA
• USDA-NIFA Research Funding Opportunities for 2025
  Michelle Colby
• Station Accomplishments and Opportunities for Interdepartmental Collaboration
  NC229 Station Representatives

7:30 a.m. – 5:00 p.m. – Symposium Check-In/Onsite Registration

8:00 – 9:30 a.m. – Session 5: Rapid Sensing of Viral Infection and Disease Counter Measures
Moderators: Cesar Corzo and Scott Dee

• Further Evidence that Science-Based Biosecurity Provides Sustainable Prevention of PRRS Virus and Improves Productivity in Swine Breeding Herd
  Scott Dee, Pipestone, USA
• PRRSV-2 Variant Classification: A Dynamic Nomenclature for Enhanced Monitoring and Surveillance
  Kimberly VanderWaal, University of Minnesota, USA
  
• Refining Genetic Classification of Global Type 1 Porcine Reproductive and Respiratory Syndrome Virus and Characterization of their Geographic Distributions
  Jianqiang Zhang, Iowa State University, USA
• A Decadal Review of Porcine Deltacoronavirus Occurrence in U.S. Breeding Herds
  Cesar Corzo, University of Minnesota, USA
• Lightning Talks
  • Long-Term Evolutionary Dynamics of Porcine Epidemic Diarrhea Virus (PEDV) in the U.S. A Decade After Introduction
    Joao Paulo Herrera da Silva, University of Minnesota, USA
  • Time-to-Stability of Porcine Epidemic Diarrhea Virus (PEDV) and the Associated Factors in U.S. Breeding Herds
    Xiaomei Yue, University of Minnesota, USA
  • Characterizing Dead Animal Disposal Practices on Sow Farms and Assessing PRRSV Risks Associated with Rendering
    Igor Paploski, University of Minnesota, USA
  • Use of Post-Mortem Samples from Growing Pigs to Detect Five Swine Pathogens
    Marcello Melini, University of Minnesota, USA
  • Isolation and Characterization of Porcine Sapovirus Genogroup III
    Ethan Aljets, Iowa State University, USA
  • Comparison of Historical and Contemporary Field Isolates of Porcine Hemagglutinating Encephalomyelitis Virus
    Trevor Arunsiripate, Iowa State University, USA

9:30 – 10:00 a.m. – Break

10:00 – 11:30 a.m. – Session 6: SHIC Special Session: Novel Tools and Technologies to Address Emerging Diseases of Swine
Moderators: Megan Niederwerder and Lisa Becton

• Development of a Standardized Outbreak Investigation Program Web-Based Application
  Derald Holtkamp, Iowa State University, USA
• Optimizing Tongue-Tip Sampling Protocols for Enhanced PRRS Virus Isolation
  Onyekachukwu (Henry) Osemeke, Iowa State University, USA
• Evaluation of Deployable Fan Coverings for Biocontainment of Airborne Swine Pathogens
  Erin Kettelkamp, Swine Vet Center, USA
• Evaluation of Electrostatic Precipitation for Biocontainment of Viral and Bacterial Pathogens Emitted from Finishing Facilities
  Brett Ramirez, Iowa State University, USA
• Self-Vaccinating Pigs to Save Labor, Improve Efficacy and Enhance Biosecurity
  Lucas Spetic Da Selva, Texas Tech University, USA
• Development of an Online Dashboard for Near-Real Time Global Swine Disease Surveillance
  Rachael Schulte, University of Minnesota, USA

11:30 a.m. – 1:30 p.m. – Session 7: Industry Sponsor Lunch Session: Current and Future Technologies in Swine Health
Moderators: Rolf Rauh and Leyi Wang

• 11:30 a.m. – 12:15 p.m.: Food Service
• History and Future of Virus Receptor Modification for the Prevention of PRRS and Other Swine Diseases
  Raymond (Bob) Rowland, University of Illinois, USA
• Efficacy Study Evaluating INGELVAC PRRS MLV Against a PRRSv ‘144’ Lineage-1C.5 Variant Heterologous Challenge
  Reid Phillip, Boehringer Ingelheim Animal Health, USA
• Think Big! Maximize your Samples with Power of Multiplexing
  Sineej Madathil, MilliporeSigma, USA
• Spatial Transcriptomics: A New Frontier for Swine Viral Pathobiology Research
  Laura Miller, Kansas State University Diagnostic Laboratory, USA

1:30 – 3:30 p.m. – Poster Session
See “Poster Presentations” for a list of presentations.

3:30 – 5:00 p.m. – Session 8: Emerging Swine Viral Diseases and Global Disease Control
Moderators: Mariana Kikuti and Jishu Shi

• Emergence of PRRSV-1 Strains of Enhanced Virulence in Europe: Overview and Evolution
  Enric Mateu, Universitat Autònoma de Barcelona Spain
• Assessing the Relative Importance of Geographic Proximity and Pig Movement-Related Factors to the Spread of PRRSV-1 in the United Kingdom
  Jean-Pierre Frossard, Animal and Plant Health Agency, UK
• Of Pigs and Men: The Best-Laid Plans for Prevention and Control of African Swine Fever
  Jishu Shi, Kansas State Univeristy, USA
• PRRS Virus Variant 1H.18: Occurrence and Classification Challenges
  Mariana Kikuti, University of Minnesota, USA
• Emerging Porcine Circoviruses
  Pablo Pineyro-Pineiro, Iowa State University, USA

5:15 – 6:15 p.m. – Break

6:15 – 9:00 p.m. – Banquet and Award Ceremony

• 6:15 – 7:15 p.m.: Food Service
• 7:15 – 8:00 p.m.: Influenza A Virus in Swine: The Expanding Problem
  Andrew Bowman, The Ohio State University, USA
• 8:00 – 9:00 p.m.: Award Ceremony
  • 8:15 – 8:15 p.m.: Thirty years of PRRS Research in my Rearview Mirror
    Jay Calvert, Zoetis Animal Health, USA
  • 8:15 – 8:30 p.m.: PRRS Research – Importance of Teamwork
    Joan Lunney, USDA ARS BARC, USA
  • 8:30 – 9:00 p.m.: Graduate Student Award Announcement

Poster session will be held Monday, December 9, from 1:30 – 3:30 p.m.

Section 1: Virus Structure and Gene Function

• #01-01  Role of N-glycosylation in PRRSV Infection
  Alberto Brandariz Nunez
• #01-02  An Intra-Family Conserved High-Order RNA Structure Within the M ORF is Important for Arterivirus Subgenomic RNA Accumulation and Infectious Virus Production
  Chi Chen
• #01-03  Cross-Species Analysis of CD163 Orthologs Reveals Key Domain for PRRSV Susceptibility
  Kassandra Durazo-Martinez
• #01-04  PRRSV nsp1α Differentially Regulates Viral RNA Synthesis
  Yanhua  Li
• #01-05  A Novel Reverse Genetics System for Porcine Reproductive and Respiratory Syndrome Virus and the Generation of a Transcription Network-Reprogrammed Virus
  Junyu Tang
• #01-06  Segment-Specific Enteroids from Pig Small Intestine in Matrigel and Transwell Inserts: Susceptibility to Porcine Epidemic Diarrhea Virus (PEDV)
  Lu Yen

Section 2: Viral Infection and Host Responses      

• #02-01  Comparison of Historical and Contemporary Field Isolates of Porcine Hemagglutinating Encephalomyelitis Virus
  Trevor Arunsiripate
• #02-02  Computationally Predicted T-cell Epitope Trends for 30 Years of Wild-Type PRRSV-2 Strains from the USA
  Julia Baker
• #02-03  Coinfection with PEDV and BVDV Induces Inflammatory Bowel Disease Pathway Highly Enriched in PK-15 Cells
  Jinghua Cheng
• #02-04  Deconstructing the Role of SYNGR2 in Viral Disease Susceptibility in Swine
  Daniel Ciobanu
• #02-05  Understanding Host X Pathogen Associations In Swine Infectious Diseases
  Daniel Ciobanu
• #02-06  Defining Interferon Antagonism Hierarchy of Porcine Epidemic Diarrhea Virus
  Xufang Deng
• #02-07  Longitudinal Assessment of Associations Between Mucosal Microbial Communities and Porcine Respiratory and Reproductive Syndrome Virus Pathogenesis
  Luoyan Duan
• #02-08  Porcine Antibody Response to Epitope A on PRRSV GP5 and Its Role in Virus Neutralization
  Jing Huang
• #02-09  scRNAseq Analysis of PBMCs During Acute PRRSV Infection: Searching for Host Cellular Markers to Predict Persistent Infection
  Mehak Kapoor
• #02-10  Evaluation of PCV3 Humoral Responses in Experimentally Infected Pigs and Dynamic of Maternally Derived Antibodies in Piglets from Naturally Infected Sows
  Molly Kroeger   
• #02-11  Single Cell T cell Receptor Profiling in Pig Lungs Using Single-Cell TCR Sequencing Analysis
  Darling Melany Madrid
• #02-12  The Absence of CD163 Lessens the Pathogenicity of PCV2d in Pigs
  Raymond (Bob) Rowland
• #02-13 Investigating MAIT Cell Contributions to Anti-Influenza Virus Immunity Using MR1 Knockout Pigs
  Laurie Touchard
• #02-14  Investigation of Fetal Liver, Heart, and Thymus Transcriptomes for Prediction of Reproductive Failure
  Kristen Walker
• #02-15 Generation of ANP32A Knock-out Cell Line for Functional Assessment of Novel Swine Isoform on Avian Influenza Virus Polymerase Activity
  Lianna Walker
• #02-16 Transduction Efficiency of HIV-Based Lentiviral Vector in Three Different Porcine Cell Lines
  Xiuqing Wang
• #02-17  CRISPR-Cas9 Mediated Identification of Host Factors for Influenza Infection and Persistence -Progress Report
  Xiuqing Wang
• #02-18  Functional Mechanism of Porcine Epidemic Diarrhea Virus Nucleocapsid Protein in Evading from Host Interferon Immune Responses
  Jidong Xu
• #02-19  A Plausible Mechanism Responsible for the Variation of PRRS Virus Virulence
  Federico Zuckermann

Section 3: Vaccination Strategies and Therapeutics

• #03-01  PRRSV-2 Immune Biobank for Vaccine Efficacy Prediction
  Elisa Crisci
• #03-02  Evaluation of a Stable Oil-In-Water Adjuvant for Recombinant PRRSV Vaccine
  Marie-Eve Koziol
• #03-03  Assessment of Homologous and Heterologous PCV2 Vaccine Efficacy in a PCV2d/PRRSV Co-Challenge Model
  Molly Kroeger
• #03-04 Comparison of Economic Losses Between Status 3 and Status 2vx Farrow to Wean Farms Equipped with Air Filtration Facilities Within 12 Weeks Following the Introduction of PRRSV NADC30-Like Strain
  Reid Philips
• #03-05  Efficacy of an Intranasal Naturally Attenuated Live PRRSV-2 Vaccine Against a Highly Virulent PRRSV-1 Strain
  Enric Mateu
• #03-06  Development of a Foot-and-Mouth Disease Virus Vaccine Candidate: Utilizing Non-Toxic Enterotoxin and E. coli as an Adjuvant-Delivery System
  Akhila Naru
• #03-07  Lipid Nanoparticle-Encapsulated DNA Vaccine Encoding African Swine Fever Virus p54 Antigen Elicits Robust Immune Responses in Pigs
  The Nguyen
• #03-08  Experimental Evidence of Vaccine-Driven Evolution of PRRSV-2 in Pigs-To-Pig Infection Chains
  Nakarin Pamornchainavakul
• #03-09  The Efficacy Study Evaluating INGELVAC PRRS® MLV Against a PRRSV ‘144’ Lineage-1C.5 Variant Challenge
  Reid Philips
• #03-10  Development and In Vitro Characterization of Messenger RNA-Based Vaccines Against Senecavirus A
  Kepalee Saeng-Chuto
• #03-11  A Live-Attenuated Virus Vaccine Candidate Protects Pigs Against Contemporary Pandemic Genotype II African Swine Fever (ASF) Virus
  Jishu Shi
• #03-12  Lipid Nanoparticle-Encapsulated DNA Vaccines: A Versatile Platform for Rapid Development of Vaccines Against Influenza A Viruses of Swine
  Hiep Vu
• #03-13  Rational Design and Immunogenicity Evaluation of mRNA-based Vaccine for African Swine Fever Virus
  Fangfeng Yuan
• #03-14  An Effective Vaccine Against Swine Influenza A Virus Based on the Matrix Protein 2 (M2)
  Federico Zuckermann

Section 4: Diagnostics, Surveillance, and Disease Control           

• #04-01  Isolation and Characterization of Porcine Sapovirus Genogroup III
  Ethan Aljets
• #04-02  BioAerium: A Real-Time Detector of Airborne Animal Viruses
  Michael Caffrey
• #04-03  Further Evidence that Science-Based Biosecurity Prevents Porcine Reproductive and Respiratory Syndrome Virus Infection and Improves Productivity in Breeding Herds
  Scott Dee
• #04-04  Assessing the Relative Importance of Geographic Proximity and Pig Movement-Related Factors to the Spread of PRRSV-1 in the United Kingdom
  Jean-Pierre Frossard
• #04-05  Long-Term Evolutionary Dynamics of Porcine Epidemic Diarrhea Virus (PEDV) in the U.S. a Decade After Introduction
  Joao Paulo Herrera da Silva
• #04-06  A Decadal Review of Porcine Deltacoronavirus Occurrence in U.S. Breeding Herds
  Mariana Kikuti
• #04-07  PRRS Virus Variant 1H.18: Occurrence and Classification Challenges
  Mariana Kikuti
• #04-08  Isolation and Characterization of Novel Reassortant Mammalian Orthoreovirus from Pigs in the United States
  Wenjun Ma
• #04-09  Think Big! Maximize Your Samples with Power of Multiplexing
  Sineej Madathil
• #04-10  Use of Post-Mortem Samples from Growing Pigs to Detect Five Swine Pathogens
  Marcello Melini
• #04-11 Assessment of farm Surface PRRSV Contamination Through Viability RT-qPCR
  Marcello Melini
• #04-12  Spatial Transcriptomics: A New Frontier for Swine Viral Pathobiology Research
  Laura Miller
• #04-13  Characterizing Dead Animal Disposal Practices on Sow Farms and Assessing PRRSV Risks Associated with Rendering
  Igor Paploski
• #04-14  Maximizing PRRSV Diagnostic Accuracy: Insights from Tongue Tip Testing
  Igor Paploski
• #04-15  An Automated 384-Well RT-qPCR Assay for Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Enhances High-Volume Testing Capabilities in the Veterinary Diagnostic Laboratory
  Bala Mounika Reddi
• #04-16  Of Pigs and Men: The Best -Laid Plans for Prevention and Control of African Swine Fever
  Jishu Shi
• #04-17  PRRSV-2 Variant Classification: A Dynamic Nomenclature for Enhanced Monitoring and Surveillance
  Kimberly VanderWaal
• #04-18  Investigating the Current Practice in Oral Fluid Sampling in the U.S. Swine Industry: An Exploratory Study
  Xiaomei Yue
• #04-19  Time-to-Stability of Porcine Epidemic Diarrhea Virus (PEDV) and the Associated Factors in U.S. Breeding Herds
  Xiaomei Yue
• #04-20  Refining Genetic Classification of Global Type 1 Porcine Reproductive and Respiratory Syndrome Virus and Characterization of Their Geographic Distributions
  Jianqiang Zhang
• #04-21  Comparison of Economic Losses Between Status 3 and Status 2vx Farrow to Wean Farms Equipped With Air Filtration Facilities Within 12 Weeks Following the Introduction of PRRSV NADC30-like Strain
  Kangning Zhao