
Recent Publications
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Recent Publications
(* corresponding author)
- Upadhyay I, SM Parvej, Y. Shen, S Li, KL Lauder, C. Zhang, W. Zhang*. 2023. Protein-based vaccine candidate MecVax broadly protects against intestinal colonization of ETEC strains expressing different adhesins (CS1 – CS6) in a rabbit model. Infect Immun. e00316-23, Accepted.
- Li, S, S Anvari, G Ptacek, I Upadhyay, RW Kaminiski, DA Sack, W Zhang*. 2023. A broadly immunogenic polyvalent Shigella multiepitope fusion antigen (MEFA) protein protects against S. sonnei and S. flexneri lethal pulmonary challenges in mice. Infect Immun. IAI, e00090-23.
- Li, S, H Seo, I Upadhyay, W Zhang*. 2023. A polyvalent adhesin-toxoid multiepitope-fusion-antigen (MEFA-IIb) induced functional antibodies against five enterotoxigenic Escherichia coli adhesins (CS7, CS12, CS14, CS17, and CS21) but not enterotoxins (LT, STa). Microorganisms. 2023, 11:2473.
- I Upadhyay, Parvej SM, S Li, KL Lauder, Y Shen, W Zhang*. 2023. Polyvalent protein adhesin MEFA-II induces functional antibodies against enterotoxigenic Escherichia coli (ETEC) adhesins CS7, CS12, CS14, CS17, and CS21 and heat-stable toxin (STa). Appl Environ Microbiol 89(6):e00683.
- Upadhyay I, S Li, H Seo, DA Sack, W Zhang*. 2022. A polyvalent protein antigen and rabbit colonization and infant passive protection models for a multivalent cholera vaccine. Proc Natl Acad Sci USA, 119(50):e2202938119.
- Upadhyay I, KL Lauder, S Li, G Ptacek, W Zhang*. 2022. Intramuscularly administered enterotoxigenic Escherichia coli (ETEC) vaccine candidate MecVax prevented H10407 intestinal colonization in an adult rabbit colonization model. Microbiol Spect 10(4):e0147322.
- Li, S, X Han, I Upadhyay, W. Zhang*. 2022. Characterization of functional B-cell epitopes at the amino terminus of Shigella invasion plasmid antigen B (IpaB). Appl Environ Microbiol 88(15):e0038422.
- Seo H, Q Duan, I Upadhyay, W Zhang*. 2022. Evaluation of multivalent enterotoxigenic E. coli vaccine candidate MecVax antigen dose-dependent effect in a murine model. Appl Environ Microbiol 88(17):e0095922.
- Kyle D. Brumfield, Hyesuk Seo, Nne Idegwu, Chad Artman, Weiping Zhang, David Sack, Julius Goepp. 2022. Feasibility of Avian Antibodies as Prophylaxis Against Diarrheal Illness. Front Immun 13:e1011200.
- Garcia YC, H Seo, DA Sack, W. Zhang*. 2022. Intradermally administered enterotoxigenic E. coli vaccine candidate MecVax induces functional serum IgG antibodies against seven adhesins (CFA/I, CS1-CS6) and both toxins (STa, LT). Appl Environ Microbiol, 88(4):e0213921.
- Jones R, H Seo, W Zhang, DA Sack. 2022. A multi-epitope fusion antigen (MEFA) candidate vaccine for ETEC is protective against strain B7A colonization in a rabbit model. PLOS Negl Trop Dis, 16(2):e0010177.
- Li S, KH Lee, W Zhang*. 2022. Multiepitope fusion antigen: MEFA, an epitope- and structure-based vaccinology platform for multivalent vaccine development. Methods Mol Biol 2412:151-169.
- Seo H, C Garcia, XS Ruan, QD Duan, DA Sack, W Zhang*. 2021. MecVax, a novel structure- and epitope-based multivalent enterotoxigenic E. coli vaccine for broad protection against ETEC-associated diarrhea. Infect Immun 89(7):e00106-21 (Spotlight Selection/Vaccines).
- Cox E, M Aloulou, J Fleckenstein, C Schäffer, Å Sjöling, S Schuller, K Hanevik, B Devriendt, W Zhang, A M Svennerholm, E Dudley. 2020. The intriguing interaction of Escherichia coli with the host environment and innovative strategies to interfere with colonization: A summary of the 2019 E. coli and the Mucosal Immune System meeting. Appl. Environ. Microbiol. 86(24):e2085-20.
- Seo H, QD Duan, W Zhang*. 2020. Vaccines against gastroenteritis, current progress and challenges. Gut Microbes 11(6):1487-1517.
- Duan QD, S Pang, W Wu, B Jiang, W Zhang, S Liu, X Wang, Z Pan, G Zhu. 2020. A multivalent vaccine candidate targeting enterotoxigenic Escherichia coli fimbriae for broadly protecting against porcine post-weaning diarrhea. Vet. Res. 51(1):93.
- Lu T, RA Rodney, W. Zhang*. 2020. Immunogenicity characterization of a fimbria-toxin multiepitope fusion antigen (MEFA) of enterotoxigenic Escherichia coli (ETEC) causing post-weaning diarrhea (PWD) in pigs. Appl. Environ. Microbiol. 86(24): AEM00274-20.
- Duan QD, W Wu, Z Pan, S Pang, W Zhang, G Zhu*. 2020. Co-administered two enterotoxigenic Escherichia coli (ETEC) fimbriae MEFA (multiepitope fusion antigen) induce neutralizing antibodies against five ETEC fimbriae: F4, F5, F6, F18 and F41. Appl. Environ. Microbiol. 86(24):AEM.00217-20.
- Ou, BM; B Jiang, J Duo; Y Yang, MY Zhang, D Zhang, HZ Zhao, MX Xu, HL Song, WW Wu, ML Chen, T Lu, JC Huang, H Seo, C Garcia, WL Zheng, WY Guo, YH Lu, S Yang, R Kaushik, XC Li, W Zhang*, GQ Zhu*. 2020. Engineered recombinant Escherichia coli probiotic strains integrated with F4 and F18 fimbriae cluster genes in the chromosome and their assessment of immunogenic efficacy in vivo. ACS Synth Biol. 9(2):412-426.
- Seo H, W Zhang*. 2020. Development of effective vaccines for enterotoxigenic Escherichia coli. Lancet Infect. Dis. 20(2):150-152.
- Seo H, T. Lu, S. Mani, AL Bourgeois, R Walker, DA Sack, W. Zhang*. 2020. Adjuvant dose effect of enterotoxigenic Escherichia coli (ETEC) double mutant heat-labile toxin (dmLT) on upimmunoregulating CFA/I/II/IV MEFA for antibody responses to seven ETEC adhesins (CFA/I, CS1-CS6). Hum Vaccin Immunother 16(2):419-425.
- Duan QD, P Xia, RM Nandre, W. Zhang*, GQ Zhu*. 2019. A review of newly identified functions associated with heat-labile toxin of enterotoxigenic Escherichia coli. Front. Cell. Infect. Microbiol. 9: e00292.
- Seo H, MR Nandre, J. Nietfeld, Z Chen, J. Huang, QD Duan, W. Zhang*. 2019. Antibodies derived from enterotoxigenic Escherichia coli (ETEC) adhesin major structural subunit antigen and minor tip subunit antigen equivalently inhibited bacteria in vitro adherence. PLoS ONE 14(5):e0216076.
- Lu T, R. Moxley, W. Zhang*. 2019. Mapping the neutralizing epitopes of enterotoxigenic Escherichia coli (ETEC) K88 (F4) fimbrial adhesin and major subunit FaeG. Appl. Environ. Microbiol. 85(11):e00329.
- Lu T, H. Seo, RA Rodney, W. Zhang*. 2019. Mapping the neutralizing epitopes of F18 fimbrial adhesin subunit FedF of enterotoxigenic Escherichia coli (ETEC). Vet. Microbiol. 230:171-177.
- Seo H, T. Lu, RM Nandre, Q. Duan, W. Zhang*. 2019. Immunogenicity characterization of genetically fused or chemically conjugated enterotoxigenic Escherichia coli (ETEC) heat-stable toxin (STa) toxoids in mice and pigs. FEMS Microbiology Lett. 366(4):fnz037.
- Huang J, QD Duan, and W Zhang*. 2018. The significance of enterotoxigenic Escherichia coli (ETEC) heat-labile toxin (LT) enzymatic subunit epitopes in LT enterotoxicity and immunogenicity. Appl. Environ. Microbiol. 84(15):e00849-18.
- Duan QD, T Lu, C Garcia, C Yanez, RM Nandre, DA Sack, W Zhang*. 2018. Co-administered tag-less toxoid fusion 3xSTaN12S-mnLTR192G/L211A and CFA/I/II/IV MEFA (multiepitope fusion antigen) induce neutralizing antibodies to 7 adhesins (CFA/I, CS1-CS6) and both enterotoxins (LT, STa) of enterotoxigenic Escherichia coli (ETEC). Frontiers in Microbiology, 9:e1198.
- Nandre R, XS Ruan, T Lu, DA Sack, W Zhang*. 2018. Enterotoxigenic Escherichia coli (ETEC) adhesin-toxoid MEFA (multiepitope fusion antigen) CFA/I/II/IV-3xSTaN12S-mnLTG192G/L211A derived antibodies inhibit adherence of seven adhesins (CFA/I, CFA/II & CFA/IV), neutralize enterotoxicity of both toxins (LT & STa), and protect piglets against diarrhea. Infect. Immun. 86(3):e00550-17.
- Duan QD, JC Huang, N Xiao, H Seo, W Zhang*. 2018. Neutralizing anti-STa antibodies derived from enterotoxigenic Escherichia coli (ETEC) toxoid fusions with heat-stable toxin (STa) mutant STaN12S, STaL9A/N12S or STaN12S/A14T show little cross-reactivity with guanylin or uroguanylin. Appl. Environ. Microbiol. 84(2): e01737-17).
- Ou BM, C Garcia, YJ Wang, W Zhang*, GQ Zhu*. 2018. Techniques for chromosomal integration and expression optimization in Escherichia coli. Biotechnology & Bioengineering. 115:2467-2478.