Publications

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Recent Publications

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Recent Publications

(* corresponding author)

  1. Upadhyay I, SM Parvej, Y. Shen, S Li, KL Lauder, C. Zhang, W. Zhang*.  2023.  Protein-based vaccine candidate MecVax broadly protects against intestinal colonization of ETEC strains expressing different adhesins (CS1 – CS6) in a rabbit model. Infect Immun. e00316-23, Accepted. 
  2. Li, S, S Anvari, G Ptacek, I Upadhyay, RW Kaminiski, DA Sack, W Zhang*.  2023.  A broadly immunogenic polyvalent Shigella multiepitope fusion antigen (MEFA) protein protects against S. sonnei and S. flexneri lethal pulmonary challenges in mice.  Infect Immun. IAI, e00090-23. 
  3. Li, S, H Seo, I Upadhyay, W Zhang*.  2023.  A polyvalent adhesin-toxoid multiepitope-fusion-antigen (MEFA-IIb) induced functional antibodies against five enterotoxigenic Escherichia coli adhesins (CS7, CS12, CS14, CS17, and CS21) but not enterotoxins (LT, STa).  Microorganisms.  2023, 11:2473. 
  4. I Upadhyay, Parvej SM, S Li, KL Lauder, Y Shen, W Zhang*.  2023.  Polyvalent protein adhesin MEFA-II induces functional antibodies against enterotoxigenic Escherichia coli (ETEC) adhesins CS7, CS12, CS14, CS17, and CS21 and heat-stable toxin (STa).  Appl Environ Microbiol 89(6):e00683. 
  5. Upadhyay I, S Li, H Seo, DA Sack, W Zhang*.  2022.  A polyvalent protein antigen and rabbit colonization and infant passive protection models for a multivalent cholera vaccine.  Proc Natl Acad Sci USA, 119(50):e2202938119. 
  6. Upadhyay I, KL Lauder, S Li, G Ptacek, W Zhang*.  2022.  Intramuscularly administered enterotoxigenic Escherichia coli (ETEC) vaccine candidate MecVax prevented H10407 intestinal colonization in an adult rabbit colonization model.  Microbiol Spect 10(4):e0147322. 
  7. Li, S, X Han, I Upadhyay, W. Zhang*.  2022. Characterization of functional B-cell epitopes at the amino terminus of Shigella invasion plasmid antigen B (IpaB).  Appl Environ Microbiol 88(15):e0038422. 
  8. Seo H, Q Duan, I Upadhyay, W Zhang*.  2022.  Evaluation of multivalent enterotoxigenic E. coli vaccine candidate MecVax antigen dose-dependent effect in a murine model. Appl Environ Microbiol 88(17):e0095922. 
  9. Kyle D. Brumfield, Hyesuk Seo, Nne Idegwu, Chad Artman, Weiping Zhang, David Sack, Julius Goepp. 2022. Feasibility of Avian Antibodies as Prophylaxis Against Diarrheal Illness.  Front Immun 13:e1011200. 
  10. Garcia YC, H Seo, DA Sack, W. Zhang*.  2022.  Intradermally administered enterotoxigenic E. coli vaccine candidate MecVax induces functional serum IgG antibodies against seven adhesins (CFA/I, CS1-CS6) and both toxins (STa, LT).  Appl Environ Microbiol, 88(4):e0213921. 
  11. Jones R, H Seo, W Zhang, DA Sack.  2022. A multi-epitope fusion antigen (MEFA) candidate vaccine for ETEC is protective against strain B7A colonization in a rabbit model.  PLOS Negl Trop Dis, 16(2):e0010177. 
  12. Li S, KH Lee, W Zhang*.  2022.  Multiepitope fusion antigen: MEFA, an epitope- and structure-based vaccinology platform for multivalent vaccine development.  Methods Mol Biol 2412:151-169. 
  13. Seo H, C Garcia, XS Ruan, QD Duan, DA Sack, W Zhang*.  2021. MecVax, a novel structure- and epitope-based multivalent enterotoxigenic E. coli vaccine for broad protection against ETEC-associated diarrhea.  Infect Immun 89(7):e00106-21 (Spotlight Selection/Vaccines). 
  14. Cox E, M Aloulou, J Fleckenstein, C Schäffer, Å Sjöling, S Schuller, K Hanevik, B Devriendt, W Zhang, A M Svennerholm, E Dudley.  2020.  The intriguing interaction of Escherichia coli with the host environment and innovative strategies to interfere with colonization:  A summary of the 2019 E. coli and the Mucosal Immune System meeting.  Appl. Environ. Microbiol.  86(24):e2085-20. 
  15. Seo H, QD Duan, W Zhang*.  2020.  Vaccines against gastroenteritis, current progress and challenges.  Gut Microbes 11(6):1487-1517. 
  16. Duan QD, S Pang, W Wu, B Jiang, W Zhang, S Liu, X Wang, Z Pan, G Zhu.  2020. A multivalent vaccine candidate targeting enterotoxigenic Escherichia coli fimbriae for broadly protecting against porcine post-weaning diarrhea.  Vet. Res. 51(1):93. 
  17. Lu T, RA Rodney, W. Zhang*.  2020.  Immunogenicity characterization of a fimbria-toxin multiepitope fusion antigen (MEFA) of enterotoxigenic Escherichia coli (ETEC) causing post-weaning diarrhea (PWD) in pigs.  Appl. Environ. Microbiol. 86(24): AEM00274-20. 
  18. Duan QD, W Wu, Z Pan, S Pang, W Zhang, G Zhu*. 2020.  Co-administered two enterotoxigenic Escherichia coli (ETEC) fimbriae MEFA (multiepitope fusion antigen) induce neutralizing antibodies against five ETEC fimbriae: F4, F5, F6, F18 and F41. Appl. Environ. Microbiol.  86(24):AEM.00217-20. 
  19. Ou, BM; B Jiang, J Duo; Y Yang, MY Zhang, D Zhang, HZ Zhao, MX Xu, HL Song, WW Wu, ML Chen, T Lu, JC Huang, H Seo, C Garcia, WL Zheng, WY Guo, YH Lu, S Yang, R Kaushik, XC Li, W Zhang*, GQ Zhu*. 2020.  Engineered recombinant Escherichia coli probiotic strains integrated with F4 and F18 fimbriae cluster genes in the chromosome and their assessment of immunogenic efficacy in vivo.  ACS Synth Biol. 9(2):412-426. 
  20. Seo H, W Zhang*.  2020.  Development of effective vaccines for enterotoxigenic Escherichia coli.  Lancet Infect. Dis.   20(2):150-152. 
  21. Seo H, T. Lu, S. Mani, AL Bourgeois, R Walker, DA Sack, W. Zhang*.  2020.  Adjuvant dose effect of enterotoxigenic Escherichia coli (ETEC) double mutant heat-labile toxin (dmLT) on upimmunoregulating CFA/I/II/IV MEFA for antibody responses to seven ETEC adhesins (CFA/I, CS1-CS6). Hum Vaccin Immunother 16(2):419-425. 
  22. Duan QD, P Xia, RM Nandre, W. Zhang*, GQ Zhu*.  2019.  A review of newly identified functions associated with heat-labile toxin of enterotoxigenic Escherichia coli.  Front. Cell. Infect. Microbiol. 9: e00292. 
  23. Seo H, MR Nandre, J. Nietfeld, Z Chen, J. Huang, QD Duan, W. Zhang*.  2019.  Antibodies derived from enterotoxigenic Escherichia coli (ETEC) adhesin major structural subunit antigen and minor tip subunit antigen equivalently inhibited bacteria in vitro adherence.  PLoS ONE 14(5):e0216076. 
  24. Lu T, R. Moxley, W. Zhang*.  2019.  Mapping the neutralizing epitopes of enterotoxigenic Escherichia coli (ETEC) K88 (F4) fimbrial adhesin and major subunit FaeG. Appl. Environ. Microbiol.  85(11):e00329. 
  25. Lu T, H. Seo, RA Rodney, W. Zhang*.  2019.  Mapping the neutralizing epitopes of F18 fimbrial adhesin subunit FedF of enterotoxigenic Escherichia coli (ETEC). Vet. Microbiol.  230:171-177. 
  26. Seo H, T. Lu, RM Nandre, Q. Duan, W. Zhang*.  2019. Immunogenicity characterization of genetically fused or chemically conjugated enterotoxigenic Escherichia coli (ETEC) heat-stable toxin (STa) toxoids in mice and pigs.  FEMS Microbiology Lett. 366(4):fnz037. 
  27. Huang J, QD Duan, and W Zhang*.  2018.  The significance of enterotoxigenic Escherichia coli (ETEC) heat-labile toxin (LT) enzymatic subunit epitopes in LT enterotoxicity and immunogenicity.  Appl. Environ. Microbiol. 84(15):e00849-18. 
  28. Duan QD, T Lu, C Garcia, C Yanez, RM Nandre, DA Sack, W Zhang*.  2018.  Co-administered tag-less toxoid fusion 3xSTaN12S-mnLTR192G/L211A and CFA/I/II/IV MEFA (multiepitope fusion antigen) induce neutralizing antibodies to 7 adhesins (CFA/I, CS1-CS6) and both enterotoxins (LT, STa) of enterotoxigenic Escherichia coli (ETEC).  Frontiers in Microbiology, 9:e1198. 
  29. Nandre R, XS Ruan, T Lu, DA Sack, W Zhang*.  2018.  Enterotoxigenic Escherichia coli (ETEC) adhesin-toxoid MEFA (multiepitope fusion antigen) CFA/I/II/IV-3xSTaN12S-mnLTG192G/L211A derived antibodies inhibit adherence of seven adhesins (CFA/I, CFA/II & CFA/IV), neutralize enterotoxicity of both toxins (LT & STa), and protect piglets against diarrhea.  Infect.  Immun.  86(3):e00550-17. 
  30. Duan QD, JC Huang, N Xiao, H Seo, W Zhang*.  2018. Neutralizing anti-STa antibodies derived from enterotoxigenic Escherichia coli (ETEC) toxoid fusions with heat-stable toxin (STa) mutant STaN12S, STaL9A/N12S or STaN12S/A14T show little cross-reactivity with guanylin or uroguanylin.  Appl. Environ. Microbiol.  84(2): e01737-17). 
  31. Ou BM, C Garcia, YJ Wang, W Zhang*, GQ Zhu*.  2018.  Techniques for chromosomal integration and expression optimization in Escherichia coli.  Biotechnology & Bioengineering. 115:2467-2478. 
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