{"id":33,"date":"2017-09-18T21:51:54","date_gmt":"2017-09-18T21:51:54","guid":{"rendered":"http:\/\/vetmed.illinois.edu\/ko-lab\/?page_id=33"},"modified":"2021-11-12T15:22:57","modified_gmt":"2021-11-12T15:22:57","slug":"technology","status":"publish","type":"page","link":"https:\/\/vetmed.illinois.edu\/kolaboratory\/technology\/","title":{"rendered":"Transgenic Animals"},"content":{"rendered":"

Transgenic animals<\/h3>\n

We created the following transgenic mice and made them available to the research community. These transgenic mouse lines are being transferred to the Jackson Laboratory for a wider distribution.<\/p>\n

Cyp17-iCre<\/strong>.<\/span>\u00a0 This is the first and most widely used transgenic mouse line among those created by my laboratory.\u00a0 This mouse is used for conditional deletion of genes in the androgen-producing cells and also for localizing cells that synthesize androgens.\u00a0 The generation and characterization of this line of mice was published in Genesis <\/em>in 2008.
\nReference<\/strong>: Generation of Cyp17iCre transgenic mice and their application to conditionally delete estrogen receptor alpha (Esr1) from the\u00a0ovary<\/strong>\u00a0and testis.<\/a> Bridges PJ, Koo Y, Kang DW, Hudgins-Spivey S, Lan ZJ, Xu X, DeMayo F, Cooney A,\u00a0Ko C<\/strong>. Genesis. 2008 Sep;46(9):499-505. doi: 10.1002\/dvg.20428.
\nJax Mice<\/strong>: https:\/\/www.jax.org\/strain\/028547<\/a><\/p>\n

Esr2-iCre<\/strong>.<\/span> This mouse line is used for conditional deletion of genes in the ER\u03b2 (estrogen receptor beta)-expressing cells.\u00a0 This line is also used for localizing the sites of ER\u03b2 expression.\u00a0 The generation of this line was published in Genesis <\/em>in 2016.\u00a0 A grant was submitted to NIH for the use of this model in studying estrogen receptors in the ovary.
\nReference<\/strong>: Generation of an estrogen receptor beta-iCre knock-in mouse.<\/a> Cacioppo JA, Koo Y, Lin PC, Osmulski SA, Ko CD,\u00a0Ko C<\/strong>. Genesis. 2016 Jan;54(1):38-52. doi: 10.1002\/dvg.22911.
\nJaxMice<\/strong>: https:\/\/www.jax.org\/strain\/028717<\/a><\/p>\n

Esr1-iCre<\/strong>.<\/span> This mouse line is used for conditional deletion of genes in the ER\u03b1 (estrogen receptor alpha)-expressing cells.\u00a0 This line is also used for localizing the sites of ER\u03b1 expression.\u00a0 The generation of this line is yet to be reported as a manuscript.
\nReference<\/strong>: Generation and characterization of an estrogen receptor alpha-iCre knock-in mouse.<\/a> Park CJ, Chen G, Koo Y, Lin PP, Cacioppo JA, Prohaska H, Ko CJ. Genesis. 2017 Nov 8. doi: 10.1002\/dvg.23084. [Epub ahead of print]<\/p>\n

Edn2-iCre<\/strong><\/span>.\u00a0 This mouse line is used for conditional deletion of genes in endothelin-2<\/em> expressing cells and for localizing the sites of endothelin-2<\/em> expression.\u00a0 The generation of this line of mice was published in February 2015 as a cover image <\/u>of the journal Genesis<\/em>.\u00a0 There is a high demand of this line of mice from a variety of disciplines of science.
\nReference<\/strong>: Generation and characterization of an endothelin-2 iCre mouse.<\/a> Cacioppo JA, Koo Y, Lin PC, Gal A,\u00a0Ko C<\/strong>. Genesis. 2015 Feb;53(2):245-56. doi: 10.1002\/dvg.22845.<\/p>\n

Edn2-flox.<\/strong><\/span> This mouse line is used for conditional deletion of endothelin-2<\/em> gene.\u00a0 We used this line of mice for demonstrating the critical role that endothelin-2 plays in ovulation (Scientific Reports<\/em>, 2017).\u00a0 Endothelin-2 is also expressed in the eyes, intestines and kidneys.\u00a0 This line of mice is already being requested by other laboratories.
\nReference<\/strong>: Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture.\u00a0<\/a>Cacioppo JA, Lin PP, Hannon PR, McDougle DR, Gal A,\u00a0Ko C<\/strong>. Sci Rep. 2017 Apr 11;7(1):817. doi: 10.1038\/s41598-017-00943-w.<\/p>\n<\/div>

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