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    Aditi Das

    Assistant Professor, Comparative Biosciences
    Affiliate, Department of Biochemistry, Division of Nutritional Science, Neuroscience Program, Department of Bioengineering & Beckman Institute

    Address and Contact Information

    3836 Vet Med Basic Sciences Building
    2001 S. Lincoln Avenue
    Urbana IL 61802
    Urbana, Illinois  61802

    Education

    • PostDoc, University of Illinois, Urbana Champaign
    • PhD, Princeton University
    • MSc, Indian Institute of Technology, Kanpur, India
    • BSc, Stephen's College, Delhi University, India

    Research Interests

    Metabolism of Omega-3 fatty acids and Endocannabinoids by CYP epoxygenases to yield Anti-Inflammatory Lipid Metabolites

    Our laboratory focuses on understanding how dietary fatty acids gets converted to anti-inflammatory lipid signaling molecules by epoxygenases.

    Research in our laboratory is highly interdisciplinary and collaborative. 

    Please visit Das Laboratory website

    Courses Taught

    VM 602 (Biochemistry) 

    VM603 (GI Physiology) 

    CB 449/FSHN 480 Basic Toxicology 

     

    Honors and Awards

    2017. NIH R01 Grant from NIGMS

    2016. Platform Speaker, Microsomal and Drug Oxidations

    2015. American Heart Association, National Scientist Development Grant

    2015. Lightening Talk LIPID MAPS meeting

    2014. Lightening Talk LIPID MAPS meetig

    2014. NIEHS funded travel award for International Winter Eicosanoid Conference 

    2013. Moog Lecture Series, Hauptman-Woodward Medical Research Institute

    2013. ACS Workshop Travel Award

    2012. Pew Scholar Nominee, UIUC campus

    2010. ACS Biological Division Travel Award  

    2007. Outstanding Researcher Award, NSF-NSEC.  

    2006-11. NSF-(NSEC) Postdoctoral Fellowship. 

    2005. FMC Corporation Graduate Fellowship, Princeton.  

    2004. Dean’s List Finalist for Honorific Fellowship, Princeton. 

    2001. Science and Engineering First Year Princeton Graduate Fellowship. 

    1998. CSIR-JRF Fellowship, India.  

    1993-96. Merit List Scholarship, Delhi University, India. 

     

    Awards to Students

    2015, Albert Doby award to Daniel McDougle

    2015, Fulbright Scholarship to Ms. Susan Zelasko

    2015, American Heart Association Fellowship to John Krapf

    2015, Highest Distinction Awarded to Mr. Amogh Kambalyal and Ms. Susan Zelasko

    2014, Best poster award to Ms. Susan Zelasko

    2014, American Heart Association Fellowship to Daniel McDougle

    2014, American Heart Association Fellowship to John Rouck

    2014, William and Lycman Biochemistry Award to Amogh Kambalyal

    2013, American Heart Association Fellowship to Susan Zelasko

    2013. High Distinction for Undergraduate Thesis - Christopher Mularcyzk

    2013. MCB Summer Fellowship - Susan Zelasko

     

    Research Biography

    Mechanisms of Eicosanoid Synthesizing Enzymes

     Metabolism of Omega-3 and Omega-6 fatty acids by epoxygenases in the heart

    Dietary consumption of omega-3 and omega-6 fatty acids have been linked to cardiovascular health benefits in humans. The central hypothesis is that the cardiovascular physiological effects of omega-3 and omega-6 fatty acids are partly mediated by the synthesis of eicosanoids via the epoxygenase (EPOX) pathway and also via reduction of platelet aggregation. The goal is to provide support to this central hypothesis through biochemical and physiological studies. 

     

    Novel Endocannabinoid Epoxide Metabolites

    Omega-3 and omega-6 endocannabinoids ellicit similar response to Cannabis in the body. Our lab is studying the metabolism of these endocannabinoids through the epoxygenase pathway.

    Selected Publications

    * denotes corresponding author or first author

     

     

    2017

    37. Maroutsos, D., Huff, H., and Das, A*, "N-terminal modification to increase expression of Eukaryotic P450s in E.Coli - CYP5alpha Hydroxylase and CYP2J2 epoxygenase" Methods in Molecular Biology, Book Chapter. Submitted

    36. McDougle, D.R., Abdeen,A., Adili, R., Caputo, M., Krapf, J., Johnson, R., Kilian, K., Holinstat, M., Das, A*, "Anti-inflammatory Omega-3 Endocannabinoid Epoxides", PNAS (Direct Submission), Accepted with Minor Revisions

    35. Roy, J. Pondenis, H., Fan T.*, Das, A*, "Comparative Proteomic Analysis of Metastatic and Non-metastatic Osteosarcoma of Human and Canine Origin" PLOS One, Accepted with Minor Revisions 

    32. Rouck*, J.E., Bradley*, WB.,Kambalyal, A.,Parayil, A*. and Das, A*., "Expression and Purification of cytochrome P450 taxadiene 5alpha-hydroxylase in E.Coli", Protein Expression and PurificationProtein Expr Purif. 2017 Jan 18;132:60-67. doi: 10.1016/j.pep.2017.01.008.

    2016

    33. Arnold, W., Baylon, J, Tajkorshid, E.* and Das, A.*,"Docosahexaenoic acid (DHA) preferentially binds to cardiovascular CYP2J2 and inhibits arachidonic acid (AA) metabolism" Biochemistry. 2016, 55(50):6969-6980

    32. Biochemistry, 2015, 54 (41), pp 6299–6302. Plucinski, L., Gartia, M., Arnold, W., Ameen, A., Chang, T., Hsiao, A., Liu, G.*, Das, A.*, "Substrate Binding to CYP2J2- Nanodiscs Detected by Nanoplasmonic Lygurcus Cup Arrays" Biosens Bioelectron., 2016, 337-46 

    31. Lim, SJ, McDougle, D., Das, A* and Smith, A.*, "Lipoprotein Nanoplatelets: Fluorescent, Zwitterionic Probes for Molecular and Cellular Imaging." Journal of the American Chemical Society, 2016 ASAP. 

    30. Roy, J. Pondenis, H., Fan T.*, Das, A*, "Direct Capture of Functional Proteins from Mammalian Plasma Membranes into Nanodiscs" Biochemistry, 2015, 6299–6302.

    Figure

    29. McDougle, D*., Baylon, J.* Meling D., Kambalyal, A., Tajkorshid, E.*, and Das, A*  "Incorporation of Charged Residues in the CYP2J2 F-G Loop Disrupts CYP2J2-Lipid Bilayer Interactions" Biochimica et Biophysica Acta -Biomembranes, 2015, 2060-2470.

    Image for unlabelled figure

    28.  Zelasko, S, Meling, D. and Das A*. "A critical residue that controls the isomerization of prostaglandin in thromboxane synthase", Biophysical Chemistry, 2015, 34-40.

    27. Wilcox, K., Das, A., Velasco, P., Sligar S.G. and Klein, W. "Nanoscale synaptic membrane mimetic allows unbiased high throughput screen that targets binding sites for Alzheimer’s-associated Aβ oligomers2015, PLOS ONE In Press

    26. Zelasko, S., Arnold, W., Das, A* " Endocannabinoid Metabolism by Cytochrome P450 Monooxygenases.", Prostaglandins and Other Lipid Mediators. 2015, http://www.sciencedirect.com/science/article/pii/S1098882314000744

    (News: Most downloaded article is at POLM) 

    2014 

    25. McDougle, D., Kambalayal, A., Meling, D. and Das, A*. "Endocannabinoids - Anandamide and 2- Arachidonoylglycerol are Substrates for Human CYP2J2 Epoxygenase" Journal of Pharmacology and Experimental Therapeutics, 2014, 616-27. 

    24. Meling, D., McDougle, D. and Das, A.*,"CYP2J2 epoxygenase membrane anchor plays an important role in facilitating electron transfer from CPR." Journal of Inorganic Biochemistry 2014, 47-53. 

    23. Orlando, B., McDougle, D., Lucido, M., Eng, E., Stokes, D., Das, A*., and Malkowski, M*. "Cyclooxygenase-2 Catalysis and Inhibition in Lipid Bilayer Nanodiscs" Archives of Biochemistry and Biophysics 2014, 546, 33-40. 

     

     

    22. Srivarma, S., Mularcyzk, C. Meling, D. and Das, A*. "Substrate binding thermodynamics and kinetics to thromboxane synthase in model lipid bilayers", ChemBioChem. 2014, 15, 892

     

    2013

     

    21. Zelasko S., Palaria, A., and Das, A* "Modifications to produce high-level expression of mammalian cytochrome P450s in recombinant expression systems" Protein Expression and Purification,2013,92,77-87 

     

    20. McDougle, D*., Palaria, A.*Magnetta E., Meling, D. and Das, A*. "Functional Studies of N-terminally modified CYP2J2 epoxygenase in Model Lipid Bilayers", Protein Science, 2013, 22,964 (Amrita Palaria and Daniel McDougle are equal authors)

    Figure 5

     

     

     

    19. Marty, M., Das, A., Sligar, S.G. “MALDI Mass Spectrometry of Membrane Proteins in Nanodiscs”, Analytical Bioanalytical Chemistry, 2012, Accepted.

     

    18. Das, A., Wei, Y., Pelczer, I. and Hecht, M.H. “Binding of Small Molecules to Cavity Forming Mutants of a De Novo Designed Protein”, Protein Science, 2011, 20, 702–711.

    (In News) Editor Highlight in Protein Science

     

     
    17. Turk, S., Das, A., Sligar, S. G. and Dravid, V. “Nanomechanical Detection of Cholera Toxin Using Microcantilevers Functionalized with Ganglioside-Nanodiscs” Nanotechnology, 2010, 21, 435502.

    (In News): Nanomechanical sensor can detect cholera. Nanowerk Spotlight

     

    16. Das, A. and Sligar, S.G. “Modulation of the Cytochrome P450 Reductase Redox Potential by the Phospholipid Bilayer” Biochemistry, 2009, 48(51), 12104-12.
     
     

    15. Das. A., Zhao, J. Van Duyne, R. and Sligar, S.G. “Screening of Type I and II Drug Binding to Human Cytochrome P450-3A4 in Nanodiscs by Localized Surface Plasmon Resonance Spectroscopy”,Anal. Chem., 2009, 81 (10), 3754–3759.

     

    14. Li, H., Das, A., Sibhatu, H., Jamal, J., Sligar, S. and Poulos, T.L. “Exploring the Electron Transfer Properties of Neuronal Nitric Oxide Synthase by Reversal of the FMN Redox Potential” J. Biol. Chem. 2008, 283 (50), 34762-34772.
     
     
    13. Zhao,J., Das, A. Sligar, S.G. and Van Duyne, R. “Resonance Localized Surface Plasmon Spectroscopy: Sensing Substrate & Inhibitor Binding to Cytochrome P450” J. Phys. Chem. C. 2008, 112 (34), 13084-13088.
     
     
     
    12. Gruia,F., Ionascu,D. , Kubo, M. Ye, X., Dawson, J., Osborne, R.L., Sligar, S. G., Denisov, I., Das, A. Poulos, T. L., Terner, J. and Champion, P.M. “Low Frequency Dynamics of Caldariomyces fumagoChloroperoxidase Probed by Femtosecond Coherence Spectroscopy” Biochemistry 2008, 47(18), 5156-5167.
     
     

    11. Das. A., Grinkova, Y. and Sligar, S.G. “Redox Potential Control by Drug Binding to Cytochrome P450 3A4.” J. Am. Chem. Soc. 2007, 129 (45), 13778-13779

     

    10. Das, A. and Hecht, M. H. “Peroxidase Activity of De Novo Heme Proteins Immobilized on Electrodes.” J. Inorg. Biochem. 2007, 101 (11-12), 1820-1826  
     
    9. Kimmich, N., Das. A., Sevrioukova, I, Meharenna, Y., Sligar, S.G. and Poulos, T. L. “Electron Transfer between P450cin and its FMN-Containing Redox Partner” J. Biol. Chem., 2007, 282 (37), 27006-27011
     

    8. Zhao*, J., Das, A.*, Zhang, X, Schatz, G., Sligar, S., Van Duyne, R. “Resonance Surface Plasmon Spectroscopy: Low Molecular Weight Substrate Binding to Cytochrome P450” J. Am. Chem. Soc. 2006, 128 (34), 11004-11005. (* Co-first authors)

    (In News): (1) Enhanced LSPR detects binding of small molecules. Anal. Chem., 2006, 78(21), 7356. (2) Kirrill’s Café paper of the month. August 2006

     

    7. Das, A., Trammell, S. A. and Hecht, M. H. “Electrochemical and Binding Studies of an immobilized De Novo Heme Protein” Biophy. Chem. 2006, 123 (2-3), 102-112.
     
    6. Hu, Y., Das, A., Hecht, M. H. and Scoles, G. “Nanografting De Novo Proteins onto gold surface” Langmuir 2005, 21(20), 9103-9109.
     
    5. Hecht, M.H., Das, A., Go, A., Bradley, L. and Wei, Y. (2004) “De Novo Proteins from Designed Combinatorial Libraries.” Protein Science 2004, 13(7), 1711–1723.

    4. Das, A.  “Electrochemical and Functional Studies of De Novo Alpha Helical Protein from a Designed Combinatorial Library.” Ph.D Thesis Princeton University November 2005

    3. Das, A. Model Chemistry of Aerobic Acetylene Hydratase Enzymes. M.Sc. Thesis: I.I.T (Kanpur) 1998

     

     

    Patents

    • Van Duyne, R., Zhao, J., Das, A., Zhang, X., Schatz, G. and Sligar, S. (2006) “Resonance Surface Plasmon Spectroscopy: Low Molecular Weight Substrate Binding to Cytochrome P450” Patent Applied


    • Klein, W., Sligar, S. G., Wilcox, K. Das, A. Velasco P., Marty, M. (2011) “An isolated set of central nervous system proteins that bind oligomers of amyloid beta (also known as ADDLs)” Invention Disclosure

    Grants

    NIH R01 Grant NIGMS

    American Heart Association National SDG (Role: PI)

    AHA - Grant Numbers- 15SDG25760064, 15UFEL25080175, 14PRE20130015, 14UFEL19990011, 13UFEL16890010

    Research Board (Role: PI)

    Manus Biosynthesis Industrial Funding (Role: PI)

     

     

     

     

     

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