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Yoo, Dongwan

Professor of Virology, Pathobiology
Director of Graduate Studies

Address and Contact Information

College of Veterinary Medicine
2001 South Lincoln Avenue
2217 Vet Med Basic Sciences Bldg.
Urbana, IL  61802


MRC Postdoctoral Fellow, Vaccine and Infectious Disease Organization (VIDO), Canada
PhD, University of Ottawa, Ottawa, Canada
DVM, Seoul National University, Seoul, Korea

Previous Professional Positions

Visiting Professor, Leiden University Medical Center, Leiden, The Netherlands
Associate Professor, Department of Pathobiology, University of Guelph, Canada
Assistant Professor, Department of Pathobiology, University of Guelph, Canada
MRC Post-doctoral Fellow, Veterinary Infectious Disease Organization, Canada

Research Interests

Viruses infect humans, animals, plants, insects, and microbes, and more than 90% of human illnesses are caused by viruses. Animal viruses can cross species boundaries to infect human populations, and viral infections of domestic animals can cause an enormous economic and societal impact. Virus replication relies on host cell machineries, and thus during infection continuous interaction occurs between virus and host. Virology is the study of the molecular and cellular aspects of such infections and aims to develop control measures againist infections of medical and veterinary importance. We use Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Porcine Edpidemic Diarrhea Virus (PEDV) as a model to study cell-virus interactions and to understand viral evasion strategies against host immune surveillance. We have developed a "reverse genetics" system and using this system generate genetically modified mutant viruses. This tool enables us to study various pathogenic mechanisms and to investigate roles that individual viral proteins play during infection.


  • Dr. Yoo studied Veterinary Medicine at Seoul National University in Korea. He completed his MSc in microbiology at the same institution and moved to Canada for further studies for virology in the Faculty of Health Sciences of the University of Ottawa. Following post-doctoral studies at the Vaccines and Infectious Disease Organization, Dr. Yoo was appointed Assistant Professor at Ontario Veterinary College University of Guelph where he initiated his current research on Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) as an emerged pathogen.
  • Dr. Yoo's research strengths lie in molecular virology, pathogenesis, and modern vaccinology, which forms the basis of his current research themes for two RNA viruses, an arterivirus PRRSV and a coronavirus PEDV.

Courses Taught

  • Virology and Viral Pathogenesis (PATH433/ MCB433)
  • Mechanisms Viral Pathogenesis (PATH519/ MCB561)
  • Pathobiologic Basis of Disease II (VM607)
  • Thesis Research (PATH599)
  • Seminar/Journal Club (PATH590)
  • Special Problems (PATH592)
  • Undergraduate Research (PATH 290)
  • Biochemistry Senior Thesis Research (BIOC492)
  • Research Project (MCB290)

Selected Publications

Ke, H., Lee, S., Kim, J., Liu, H.C., Yoo, D. (2019). Interaction of PIAS1 with PRRS virus nucleocapsid protein mediates NF-κB activation and triggers proinflammatory mediators during viral infection. Sci. Reports 9: 11042.

Ke, H., Han, M., Kim, J., Kurt E. Gustin, Yoo, D. (2019). Porcine reproductive and respiratory syndrome virus nsp1-beta interaction with the nucleoporin 62 facilitates virus replication in vitro. J. Virol. 93: e00469-19. (Cover page article, Volume 93, Issue 14, July 15, 2019;

Hou, Y., Ke, H. Kim, J., Yoo, D., Su, Y., Boley, P., Saif, L. J., Wang, Q. 2019. Engineering an effective and safe live attenuated PEDV vaccine candidate via inactivation of the viral 2'-O methyltransferase and the endocytosis signal of the spike protein. J. Virol. 93(15): e00406-19.

Zhang, Q., Ke, H., Blikslager, A., Fujita, T., Yoo, D. 2018. Type III interferon restriction by porcine epidemic diarrhea virus and the role of viral protein nsp1 in IRF1 signaling. J. Virol. 92(4): e01677-17.

Zhang, Q., Ma, J., Yoo, D. 2017. Inhibition of NF-κB activity by the porcine epidemic diarrhea virus nonstructural protein 1 for innate immune evaion. Virology 510: 111-126.

Han, M., Ke, H., Zhang, Q., Yoo, D. 2017.Nuclear imprisonment of host cellular mRNA by nsp1ß protein of porcine reproductive and respiratory syndrome virus. Virology 505: 42-55. (Cover page article, Volume 505, May 2017).

Sun, Y., Ke, H., Han M., Chen, N., Fang, W., Yoo, D. 2016. Nonstructural protein 11 of porcine reproductive and respiratory syndrome virus suppresses both MAVS and RIG-1 expression as one of the mechanisms to antagonize type I interferon production. PLoS ONE 11(12): e0168314. 

Hu Y, Cong X, Chen L, Qi Jm Wu X, Zhou M, Yoo D, Li F, Sun W, Wu J, Zhao X, Chen Z, Yu J, Du Y, Wang J. 2016. Synergy of TLR3 and TLR7 ligands significantly enhances function of DCs to present inactivated PRRS virus antigen through TRIF/MyD88/NF-κB signaling pathway. Sci. Reports. 6:23877. 

Zhang Q, Shi K, Yoo D. 2016. Suppression of type I interferon production by porcine epidemic diarrhea virus and degradation of CREB-binding protein by nsp1. Virology 489: 252-268.

Han M, Kim CY, Rowland RRR, Fang Y, Kim D, Yoo D. 2014. Biogenesis of non-structural protein 1 (nsp1) and nsp1-mediated type I interferon modulation in Arteriviruses. Virology 458-469:136-150.

Du Y, Bi J, Liu J, Liu X, Wu X, Jiang P, Yoo D, Zhang Y, Wu J, Wan R, Zhao X, Guo L, Sun W, Cong X, Chen L, Wang J. 2014. 3Cpro of FMDV antagonizes IFN signaling pathway by blocking STAT1/STAT2 nuclear translocation. J. Virol. 88:4908-4920.

Hicks JA, Yoo D, Liu HC. 2013. Characterization of changes in microRNA expression and function in PRRSV-infected macrophages. PLoS One 8(12): e82054. 

Du Y, Pattnaik AK, Song C, Yoo D, Li G. 2012. Glycosyl-phosphatidylinositol (GPI)-anchored membrane association of the porcine reproductive and respiratory syndrome virus GP4 glycoprotein and its co-localization with CD163 in lipis rafts. Virology 424:18-31.

Song C, Krell P, Yoo D. 2010. Nonstructural protein 1a subunit-based inhibition of NF-kB activation and suppression of interferon-ß production by porcine reproductive and respiratory syndrome virus. Virology 407:268-280.

Kim O, Sun Y, Lai FW, Song C, Yoo D.2010. Modulation of type I interferon induction by porcine reproductive and respiratory syndrome virus and degradation of CREB-binding protein by non-structural protein 1 in MARC-145 and HeLa cells. Virology 402:315-326.  

Pei Y, Hodgins DC, Wu J, Welch SKW, Calvert JG, Li G, Du Y, Song C, Yoo D. 2009. Porcine reproductive and respiratory syndrome virus as a vector: Immunogenicity of green fluorescent protein and porcine circovirus type-2 capsid expressed from dedicated subgenomic RNAs. Virology 389:91-99.

Song C, Lu R, Bienzle D, Liu HC, Yoo D. 2009. Interaction of porcine reproductive and respiratory syndrome virus nucleocapsid protein with the inhibitor of MyoD family-a domain containing protein. Biol. Chem. 390:215-223.

Lee C, Hodgins DC, Calvert JG, Welch SK, Jolie R, Yoo D. 2006. Mutations in the nuclear localization signal of the porcine reproductive and respiratory syndrome virus nucleocapsid protein attenuate virus replication. Virology 346:238-250 (Cover page article, Volume 346 Issue 1).

Lee C, Calvert JG, Welch SK, Yoo D. 2006. A DNA-launched reverse genetic system for porcine reproductive and respiratory syndrome virus reveals that homodimerization of the nucleocapsid protein is essential for virus infectivity. Virology 331:47-62.

Yoo D, Wootton S, Li G, Song C, Rowland RR. 2003. Co-localization and interaction of the porcine arterivirus nucleocapsid protein with the small nucleolar RNA-associated protein fibrillarin. J. Virol. 77:12173-12183.

Wootton S, Yoo D. 2003. Homo-oligomerization of the porcine reproductive and respiratory syndrome virus nucleocapsid protein and the role of disulfide linkages. J. Virol. 77: 4546-4557.

Wootton S, Rowland RR, Yoo D.2002. Phosphorylation of the porcine reproductive and respiratory syndrome virus nucleocapsid protein. J. Virol. 76:10569-10576.

Current Projects

  • Immune Modulation and Evasion Strategy of PRRS Virus
  • PRRS Virus as a Multivalent Vaccine Vector
  • Molecular Bases for Interferon Modulation by PRRS Virus and PEDV


  • USDA NIFA 2018-2022 PRRSV regulation for inflammatory regulators (PI)
  • USDA NIFA 2013-2018 Immune Evasion of PRRSV and Vaccine Design (PI)
  • USDA CSREES-NRI 2008-2012, Immune Modulation of PRRSV (PI)
  • USDA HATCH, PRRS Virus as a Multivalent Vaccine Vector (PI)
  • NIH T32, Infection Biology Training Program (Co-PI)
  • NIH T35, Summer Training Translational Biomedical Research (Co-PI)

Honors and Awards

  • Gordon and Helen Kruger Research Excellence Award (2018)
  • Pfizer Award for Research Excellence (2012)
  • Presidential Distinguished Professor Award (Research) (2003)
  • Pfizer Faculty Award for Research Excellence (2003)
  • Honorary Senior Scientist, Korean Ministry of Agriculture, Rural Development Administration (1992-2003)

Professional Affiliations

  • Editorial Board - Virology (Elsevier)
  • Editorial Board - Virus Research (Elsevier)
  • Editorial Board - Animal Health Research Review (Cambridge University Press)
  • Associate Editor - Journal of Veterinary Science (Korean Society of Veternary Science)
  • American Society for Virology (life-time member)
  • American Society for Microbiology (member)
  • Conference of Research Workers in Animal Diseases (member)

Service Activities

  • 18th International Nidovirus Symposium 2014, Scientific Program Committee, Spain
  • International Committee on Taxonomy of Viruses, Arterivirus Study Group 2005-2011
  • International Pig Veterinary Society Conference 2012 Scientific Committee, Korea
  • International Pig Veterinary Society Conference 2010 Scientific Committee, Canada
  • 17th International Nidovirus Symposium 2011, Scientific Program Committee, Michigan

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