Appendix C: Clinical Pathology, Veterinary Diagnostic Laboratory, College of Veterinary Medicine at the University of Illinois at Urbana-Champaign

College of Veterinary Medicine at Illinois

Veterinary Diagnostic Laboratory

Appendix C: Clinical Pathology

Chemical Profiles Endocrinology Immunohematology Chemical, Individual Function
Urinalysis Coprology Hematology Other  

* C= Centralia G=Galesburg S=State Lab U=University of Illinois

Chemical Profiles
Procedure Specimen Labs
Canine, Feline, Equine Food Animal, Canine Liver
Feline Liver, LA Liver SA Renal, LA Renal, Electrolyte		Serum, 0.5 ml U
Chemical, Individual
AlbuminSerum, 0.5 ml U
Alkaline
Phosphatase 		Serum, 0.5 ml
(fast 12 hours),chill		 C, U
AmylaseSerum, 0.5 ml U
ALT (SGPT) Serum, 0.5 ml, chill C, U
AST (SGOT) Serum, 0.5 ml, chill C, U
Bile Acids
post feeding
(2 hours)		Serum, 0.5 ml(8 hour fast.)
Serum, 0.5 ml		 U
Bilirubin, Total Serum, 0.5 ml, fast overnight, chill C, U
BUNSerum or plasma, 0.5 ml, chill  
CIAP Serum, 0.5 ml

U
CalciumSerum, 0.5 ml C, G, U 
ChlorideSerum, 0.5 ml U
CholesterolSerum 0.5 ml U
CPKSerum, 0.5 ml, chill U
CreatinineSerum, 0.5 ml C, U
Gamma GT Serum, 0.5 ml U
GlucoseSerum, 0.5 ml C, U
LipaseSerum, 0.5 ml, chill U
MagnesiumSerum, 0.5 ml C, G, U 
PhosphorousSerum, 0.5 ml C, G, U 
PotassiumSerum,0.5 ml C, U
Serum Protein Serum, 0.5 ml C, G, U 
Sodium Serum, 0.5 ml C, G, U 
Sorbitol Dehydrogenase Serum, 0.5 ml, chill U
Coprology
Fibrinogen Plasma / Whole blood, 0.5 ml U
Fibrin Split
Products		 Plasma requires special tube U
Prothrombin Time Plasma requires special tube U
Blood Gas,
Complete		 Heparinized blood,
2 ml anaerobic on ice		 U
Endocrinology
Thyroid Profile
(Total T3 / T4)		 Serum, chil / frozen 0.5 ml U
Cortisol Serum, chill / frozen, 0.5 ml U
Progesterone Serum, chill / frozen, 0.5 ml U
Testosterone Serum, chill / frozen, 0.5 ml U
TSH Serum, 0.5 ml U
Function
Procedure Specimen Labs
Baseline Check

Collect mid-morning sample for U

cortisol timing not critical for the hormones

 U
TSH Challenge

Collect pre-TSH sample

Inject 0.1 unit / kg(dogs) or 1.0 unit / kg (cats)

TSH, IV; 10 units (horses) Collect post-TSH samples 6 hours after TSH (dogs and cats); 1,2,3,4,5, and 6 hours post (horses)

U
Thyroid Replacement
Check		 Collect 6 hrs post-pill for BID therapy or 6 and 24 hrs post-pill SID U
Low-Dose
Dexamethasone Test		 Collect pre-LOW DEX sample. Inject 0.01mg/kg dexamethasone, IV. Collect post-LOW DEX sample 8 hours later, 4 hours (optional) U
High-Dose
Dexamethasone Test		 Collect pre-Hi DEX sample. Inject 1.0 mg/kg dexamethasone, IV Collect post HI-DEX sample 8 hrs later U
HCG Challenge
(for horses)		 Collect pre-ACTH sample. Inject Dogs--2 units/kg (up to a maximum of 40 units) ACTH gel, IM Horses--100 units ACTH gel, IM Collect post-ACTH sample 2 hrs later U
Pregnancy Check
(hourses only)
 Collect sample 18-24 days post-breeding U
Hematology
Buffy Coat Examination Whole blood, 2.0 ml U
CBC Whole blood, 2.0 ml & smear C, G, U 
PCV & PTP Whole blood, 1.0 ml C, U
Red Cell Count Whole blood, 1.0 ml C, G, U 
White Cell Count Whole blood, 1.0 ml C, G, U 
Hemoglobin Whole blood, 1.0 ml G, U
Differential WBC Whole blood, 2.0 ml & smear G, U
Eosinophil Count Whole blood, 2.0 ml C, U
Platelet Count Whole blood, 2.0 ml C, U
Reticulocyte Count Whole blood, 2.0 ml C, U
Smear Examination Smear (air dried, unfixed) C, G, U 
Special Stains Whole blood, 2.0 ml U
Immunohematology
Crossmatch Serum and whole blood G, U
Direct Coomb’s Whole blood U
Urine Analysis
Routine Electrolyte Clearance 10 ml U
Study (CRT,Na,K,Cl,Ca,P) 10 ml and serum U
Quantitative Protein 1.0 ml U
Protein: creatinine 1.0 ml U
Others
Electrophoresis - Serum Serum, 1.0 ml U
Electrophoresis - Urine 10 ml Urine U
Osmolarity - Serum Serum, 2.0 mls U
Osmolarity - Urine   U
Seman Evaluation Semen, 2 ml C, G, U