Appendix A: Infectious & Metabolic Diseases, Veterinary Diagnostic Laboratory, College of Veterinary Medicine at the University of Illinois at Urbana-Champaign

College of Veterinary Medicine at Illinois

Veterinary Diagnostic Laboratory

Appendix A: Infectious & Metabolic Diseases

* C= Centralia G=Galesburg S=State Lab U=University of Illinois

 

Disease Specimen Discipline Labs
Abortion (See User manual) -- -- --
Actinomycosis/
Actinobacillosis
abscess material
fixed abscess lesion
Bacteriology
Histology
C, G, U
C, G, U
Actinobacillus
pleuropneumoniae
fresh lung
fixed lung
Bacteriology
Histology
C, G, U
C, G, U
Adenovirus
infectious hepatitis
(ICH, CAVI)
fixed liver, kidney
fresh nasal and throat swabs, cerebral spinal fluid
Histology
Virology
C, G, U
C
Laryngotracheitis
(CAV2)
, canine
fixed lung, trachea, liver, kidney Histology C, G, U
Aleutian Disease fixed lymph node, spleen,liver, kidney
serum
Histology



Serology
C, G, U

C
Anthrax blood, spleen Bacteriology C, G, U
Atrophic rhinitis nasal swabs
6-8 week old pigs delivered to laboratory same day fixed nasal turbinate
Bacteriology
Histology
C, G, U


C, G, U
Black Leg
/Malignant Edema
fresh skeletal muscle
fixed skeletal muscle
Bacteriology


Histology
C, G, U


C, G, U
Blood culture inoculated blood culture system Bacteriology C, G, U
Bluetongue 10 ml heparinized blood (Green top tubes)
Serum (CF,SN,ELISA)
Virology

Serology
C

C, U
Border Disease
(BD/BVD)
Dam:rectal, nasal swabs, whole blood (heparinized preferred), serum
Lamb: 1/2 fresh brain, spinal cord, CSF,serum

1/2 fixed brain, spinal cord
Virology


Virology



Histology
C, G


C, G, U



C, G, U
Borreliosis
(canine Borrelia burgdorferi infection)
1 ml serum Serology U
Botulism Serum Bacteriology G
Bovine leukosis fixed lymph node and other involved organs
serum
Histology

Serology
G
C, U


C, U
Bovine Mammillitis fresh vesicular fluid, scrapings, biopsy of lesion, serum
fixed biopsy of lesion
Virology
Histology
U
C, G, U
Bovine Respiratory
Syncytial
fresh lung, tracheal lavage fluid
Virus fixed lung
Virology



Histology
C, G, U



C, G, U
Bovine Virus Diarrhea fresh abomasum, ileum, spleen, lymph node; fetus (kidney, liver, lung, spleen)
fixed abomasum, ileum
serum(chronic cases)
Histology

Virology
Virology
C, G, U


C, U
C, U
Bovine Virus
Diarrhea screening test
serum
(animals > 2 mon. age)

Virology U
Brucellosis

________ Livestock

________ Canine

See Abortion
heparinized blood
Serum
-----
Bacteriology
Serology
C, G, U
C, G, U
C, G, U
Campylobacter/
abortion
See Abortion ----- C, G, U
Canine Distemper fresh urinary bladder, lung, brain, conjunctival smear, heparanized blood
fixed brain, lung, urinary bladder, stomach, liver, kidney
Virology


Histology
C, G, U


C, G, U
Canine Herpesvirus fixed lung, liver, kidney, brain
fixed lung, liver, kidney, brain
Virology


Histology
C, G, U
C, G, U
Canine Parvovirus/
Coronavirus
fresh small intestine (multiple sections), feces,
fixed small intestine, spleen, mesenteric lymph nodes
Virology

Histology
C, G, U

C, G, U
Caprine Contagious Arthritis/Encephalitis fixed brain, synovial membrane, lung, mammary gland
serum
Histology

Serology
C, G, U


C, U
Caseous Lymphadenitis fresh abscessed lymph

fixed abscessed lymph node
Bacteriology

Histology
C, G, U

C, G, U
Chlamydia
______Abortion


______Avian
______Feline
See Abortion


fresh lung, liver, nasal swabs, feces


fixed lung, liver, ocular

fresh ocular, pharyngeal swabs
* fixed conjunctiva, lung
* conjunctival scrapings



Virology
Histology


Histology

Histology
Virology



C, G, U



C, G, U

G C, U

Clostridium perfringens fresh intestine, or intestinal contents

fixed duodenum, jejunum, ileum, colon
Bacteriology


Histology
C, G


C, G, U
Coccidiosis feces, intestine smears

fixed duodenum, jejunum, ileum, and colon
Parasitology

Histology
C, G, U

C, G, U
Colibacillosis

______Mammalian

______Avian
fresh duodenum, jejunum and ileum fixed duodenum, jejunum, ileum, colon

fresh liver, air sac swab

fixed liver, spleen, air sac
Bacteriology
Histology
C, G, U C, G ,U
Contagious Ecthyma
(sore mouth, ORF)
fresh skin scrapings and crusts, vesicular fluid, serum

fixed biopsy
Virology



Histology
C, U



C, G, U
Contagious
Equine Metritis
cervical secretions or male urethral fossa secretions (call laboratory for instructions) Bacteriology C
Coronavirus/Rotavirus _______Calf diarrhea _______Porcine fresh jejunum, ileum, spiral colon, intentinal contents or feces
(< 24 hours of onset)


fixed duodenum, jejunum, ileum, spiral colon

See Transmissible Gastroenteritis
Virology




Histology


C, G, U





C, G, U


Cryptosporidiosis fresh intestine, fecal or intestinal smear, feces

fixed ileum, spiral colon
Parasitology


Histology
C, G


C, G, U
Dermatophilus fresh scabs, exudate

fixed skin
Bacteriology

Histology
C, G, U

C, G, U
Dirofilariasis
(canine, feline Dirofilaria
immitis infection)
1 ml serum or plasma Serology U
Edema Disease fresh jejunum and ileum

fixed brain, stomach, spiral colon
Bacteriology


Histology
C, G, U


C, G, U
Epididymitis – Ovine semen

semen, serum
Bacteriology

Serology
C, G, U

G
Erysipelas fresh liver, kidney, spleen, lymph nodes Bacteriology C, G, U
Equine Adenovirus live: nasal and conjunctival swabs

dead: lung, parotid salivary glands, pancreas, spleen, thymus, lymph nodes fixed lung
Virology



Histology


U



C, G, U
Equine Coital
Exanthema
(EHV-3)
vesicular fluid of genital lesions Virology U
Equine Encephalomyelitis Equine Encephalomyelitis

fixed brain, spinal cord

paired serum
Virology


Histology

Serology
*


C, G, U

*
Equine Infectious Anemia fixed heart, liver, kidney, spleen

serum
Histology


Serology
C, G, U


S, U
Equine Infertility uterine swab

uterine biopsy (best if obtained at estrus)
Bacteriology

Histology
C, G, U

C, G, U
Equine Influenza nasal/pharyngeal swabs Virology C
Equine
Rhinopneumonitis
_______Abortion


===================


_______Neurologic


fresh lung and trachea, nasal/pharyngeal swabs, tracheal lavage fluid

fixed lung

paired serum

__________________
See Abortion
fresh lung, liver, spleen, kidney, thymus

fixed liver, adrenal, lung, placenta

fresh brain, spiral cord, cerebral spinal fluid

fixed brain, spinal cord
Virology




Histology

Serology

_________
Virology




Histology


Virology


Histology
C, G, U




C, G, U

C, U

____
C, G, U




C, G, U


C, G, U


C, G, U
Equine Viral Arteritis





____________Abortion
fresh lung, bronchial lymph

nodes, kidney, spleen paired serum

fresh kidney, lung, spleen
fixed liver, lung, kidney, placenta, spleen
Virology


Serology


Virology


Histology
U


C, U


U


C, G, U
Feline Calicivirus fresh nasal, ocular, pharyngeal, oral ulcer swabs, lung, or
bronchial lym
ph node, spleen
fixed lung, oral ulcers

paired serum
Virology




Histology

Serology
C, G, U




C, G, U

U
Feline Rhinotracheitis/
Herpesvirus
fresh nasal, ocular, pharyngeal, oral ulcer swabs, lung, bronchial lymph nodes, spleen

fixed lung, oral ulcers

paired serum
Virology




Histology

Serology
C, G, U




C, G, U

U
Feline Panleukopenia/
Parvovirus
fresh duodenum, jejunum, ileum

fixed jduodenum, jejunum, ileum

paired serum
Virology


Histology


Serology
C, G, U


C, G, U


U
Feline Infectious Peritonitis lung, liver, or organs w/ gross lesions

fixed brain, eye, intestine, lung, liver, kidney, spleen

serum, effusion fluids
Virology


Histology


Serology
C, G, U


C, U


C, U
Feline
Immunodeficiency Virus
serum Serology C, U
Feline Leukemia Virus serum Serology C, U
Feline Leukemia FOCMA serum Serology C, U
Glasser's Disease See porcine polyserositis ------- ----
Heartworm (canine, feline Dirofilaria immitis infection) 1 ml serum or plasma Serology C, U
Hemagglutinating Encephalomyelitis Virus fresh brain, tonsil, mandibular lymph node, lung, kidney, spleen

fixed brain
Virology


Histology
U


C, G, U
Hemophilus somnus

______Pneumonia

______TEM

fresh lung
fresh lung

fresh brain

fixed brain
Bacteriology


Histology

Bacteriology

Histology
C, G, U


C, G, U

C, G, U

C, G, U
Hemorrhagic
Bowel Syndrome
fresh affected small intestine, spiral colon

fixed affected small intestine and spiral colon
Bacteriology


Histology
C, G, U


C, G, U
Inclusion Body Rhinitis fixed nasal turbinate Histology C, G, U
Infectious Bovine
Rhinotracheitis

_________Respiratory _________Abortion

see Shipping Fever


see Abortion

-----

-----

Johne's Disease fresh feces, ileum, spiral colon, regional mesenteric lymph node

fixed ileum, spiral colon, regionalmesenteric lymph node

serum

milk
Bacteriology



Histology



Serology

Serology

C, G, U



C, G, U



C

C
Laryngotracheitis
(Herpes) Chicken
trachea Histology U, G
Leptospirosis

_______Abortion

_______Neurologic

see Abortion

fresh kidney (not frozen)

fixed kidney
------

Bacteriology

Histology
----

C, G, U

C, G, U
Listeriosis
________Abortion

________Neurologic

See Abortion

fresh 1/2 brain

fixed 1/2 brain
-------

Bacteriology

Histology
C, G, U

C, G, U

C, G, U
Lyme disease
(canine Borrelia
burgdorferi infection)
1 ml serum Serology U
Malignant
Catarrhal Fever
heparinized blood

fixed brain, lung, liver

kidney, spleen, lymph node

serum
Virology

Histology

Virology


Serology
*

C, G, U

C, G, U


*
Mastitis fresh milk; use proper sample container half full or less.

Call Lab before submitting large (>15) numbers of samples
Bacteriology



Bacteriology
C, G, U



C, G, U
Mycoplasma Arthritis fresh joint fluid or swab

fixed synovial membrane
Bacteriology

Histology
C, G, U

C, G, U
Mycoplasma hyorhinis See porcine polyserositis ----- ----
Mycoplasma
pneumonia
fresh lung

fixed lung
Bacteriology

Histology
C, G, U

C, G, U
Nocardiosis fresh involved organ or lesion
fixed lesion
Bacteriology


Histology
C, G, U


C, G, U
Ovine Progressive
Pneumonia
(Visna, Maedi)
fixed lung

serum
Histology

Serology
C, G, U

C, U
Porcine Enterovirus serum Serology C
Porcine Parvovirus fresh mummified fetus or fetal lung

paired serum
Virology


Serology
C, G, U


C, U
Porcine Polyserositis fresh meningeal, peritoneal, pleural and/or joint swabs,lung

fixed brain, heart, lung, liver, spleen
Bacteriology



Histology
C, G, U



C, G, U
Porcine Reproductive ____Respiratory Syndrome fresh lung, serum

fixed brain, heart, lung

serum
Virology

Histology

Serology
U, G

C, G, U

C, G, U
Proliferative Ileitis fixed ileum, spiral colon Histology C, G, U
Pseudo-cowpox fresh vesicular fluid, scrapings biopsy of lesion, serum
fixed biopsy
Virology

Histology
U


C, G, U
Pseudorabies fresh brain, lung, tonsils, spleen

fixed brain

serum
Virology


Histology

Serology
C, G, U


C,G,U

C, G
Rabies fresh brain Virology C, G
Rhodococcus equi fresh lung, or other affected tissue Bacteriology C, G, U
Rotavirus


________Calf


________ Porcine
See Corona Virus

fresh jejunum, ileum, fecal


fixed duodenum, jejunum, ileum, colon
----

Virology

Histology
---

C, G, U

C, G, U
Salmonellosis fresh lung, liver, jejunum, ileum, mesenteric lymph node, colon, colonic lymph node, kidney, spleen

fixed lung, liver, duodenum,jejunum, ileum, colon, kidney, spleen, lymph nodes
Bacteriology





Histology



C, G, U





C, G, U
Salt Poisoning fixed brain

serum or cerebral spinal fluid

brain, 20 g, chill
Histology

Clin Path


Toxicology
C, G, U

C, G

C, U
Semen Testing,
Bovine Viral Isolation
6 straws or equivalent per animal frozen in liquid nitrogen Virology C
Shipping Fever (Bovine)/i> __Parainfluenza(PI-3) fresh lung, trachea, ocular swabs, nasal swabs, respiratory lymph nodes, spleen

fixed lung, trachea

paired serum
fresh lung, trachea
paired serum
Bacteriology




Histology

Serolology

Virology

Serology
C, G, U




C, G, U

C, U

U

C, U
Strangles abscess exudate Bacteriology C, G, U
Swine Dysentery fresh spiral colon

fixed spiral colon
Bacteriology

Histology
C, G, U

Histology
Swine Influenza fresh lung, trachea, respiratory lymph nodes

fixed lung

paired serum
Virology


Histology

Serology

C, G, U


C, G, U

U
Systemic Fungus fresh involved organ

fixed lung, liver, other involved organs

serum
Bacteriology

Histology


Serology
C, G, U

C, G, U


U
Toxoplasmosis serum Serology C
Transmissible Gastroenteritis fresh jejunum and terminal ileum (do not freeze), diarrheic feces

fixed duodenum, jejunum, ileum, spiral colon

paired serum
Virology



Histology


Serology
C, G, U



C, G, U


C, G, U
Trichomoniasis See Abortion

uterine exudate, preputial scraping
Bacteriology C, G
Tuberculosis fresh involved tissue *

fixed involved tissue (samples will be forwarded to NVSL)
Bacteriology

Histology
G, C

C, G, U
Tyzzer's Disease fixed liver, ileum, colon Histo C, G, U
Vesicular Stomatitis fresh vesicular fluid, scrapings, biopsy, serum

fixed biopsy of lesion

serum
Virology


Histology

Serology
* , U


C, G, U

C
Weak Calf Syndrome (BVDV) fresh lung, liver, spleen, lymph node

fixed brain, heart, lung, liver, kidney, skeletal muscle

serum
Bacteriology
Virology

Histology



Serology
C, G, U


C, G, U



C, U
White Muscle Disease fixed skeletal muscle, heart, tongue

serum, liver
Histology


Chemistry
C, G, U


C, U
White Muscle Disease fixed skeletal muscle, heart, tongue

serum, liver
Histology


Chemistry
C, G, U


C, U