Illinois Department of Agriculture - Animal Disease Laboratories
Centralia - Galesburg - Springfield - University of Illinois, VDL
Laboratory User's Manual
 
User Manual


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Necropsy
Histopathology
Bacteriology
Mycology
Virology
Serology
Immunology
Toxicology
Toxicology Chemistry
Parasitology
Endocrinology
Clinical Pathology

Rabies
Reportable Diseases
Consideration

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Appendix F: Toxicologic Analysis

 



Elemental Analysis Organic Analysis
General Toxicology Analysis Special Custom Analysis

* C= Centralia G=Galesburg S=State Lab U=University of Illinois
Multielement:   Organic Analysis

Multielement Analysis
(heavy metal screen, ICP)

Includes: Ag, Al, As, B, Ba, Be, Bi, Ca, Cd,Co, Cr, Fe,K, Li, Mg, Mn, Mo,Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Tl, V, Zn Assay Specimen Labs
Tissues Carbamate Insecticide Screen Brain
__ Whole or 1/2 (longitudinal)

Liver
____ 50 g, min, freeze


Kidney
____ 50 g, min, freeze

Eyes
Stomach Contents
____ 100 g, min, freeze
Feed or Source
____ 100 g, min, freeze
Water
____ 100 ml, chill
C, U
Assay Specimen Labs

Tissues (liver, kidney)

20 g, chill / freeze U
Blood 2 ml, EDTA, chill U
Serum 2 ml, separated, chill U
Urine 2 ml, chill / freeze U
Stomach Contents 100 g, chill / freeze U
Feed or Source 100 g, chill U
Water 100 ml, chill U
Elemental Analysis Herbicide Screen   C
Assay Specimen Labs Organochlorine
Insecticide Screen
Brain
__ Whole or 1/2 (longitudinal)

Liver
____ 50 g, min, freeze


Kidney
____ 50 g, min, freeze

Stomach Contents
____ 100 g, min, freeze
Feed or Source
____ 100 g, min, freeze
Water
____ 100 ml, chill

Freeze samples promptly.

A confirmation of screen positive is necessary.

C, U
Arsenic Liver
____ 20 g, chill / freeze

Kidney
____ chill / freeze

Blood
____ 2 ml, EDTA tube, freeze

Urine
____ 2 ml, chill / freeze

Stomach Contents
____ 100 g, chill / freeze

Feed or Source
____ 100 g, chill as required

Water
____ 100 ml, chill
C, U
Cadmium Liver
____ 20 g, chill / freeze

Kidney
____ chill / freeze

Blood
____ 2 ml, EDTA tube, freeze

Urine
____ 2 ml, chill / freeze

Stomach Contents
____ 100 g, chill / freeze

Feed or Source
____ 100 g, chill as required

Water
____ 100 ml, chill
C, U Organophosphate Insecticide Screen Brain
__ Whole or 1/2 (longitudinal)

Liver
____ 50 g, min, freeze


Kidney
____ 50 g, min, freeze

Stomach Contents
____ 100 g, min, freeze
Feed or Source
____ 100 g, min, freeze
Water
____ 100 ml, chill
C, U
Vitamin A (retinol) Serum
____ 2 ml
C, U
Vitamin E Serum
____ 2 ml
C, U
Chromium Liver
____ 20 g, chill

Kidney
____ 20 g, chill

Blood
____ 2 ml, EDTA tube, freeze

Serum
____ 2 ml, seperate, chill

Feed or Source
____ 100 gm

Water
____ 100 ml, chill
C, U

Chill (if < 48 hours to the lab) or freeze samples promptly. Cholinesterase analysis is recommended first. A confirmation of screen positive samples is necessary.

Organic Compound by Request
Prior consultation with the toxicology laboratory is required before submission. A variety of organic compounds may be screened. Confirmation may require additional charges.

General Toxicology Analysis
Assay Specimen Labs
Ammonia Serum
____ 2 ml, seperated, freeze
Whole Blood
____ 2 ml, freeze
Rumen contents
____ 100 g, freeze

Sample must be frozen immediately. Thawed samples cannot be analyzed. Postmortem samples should be taken within 1 hour of death. Suspect source should be submitted for urea analysis. Seperate serum and plasma before shipping.

C, U
Copper

Liver
____ 20 g, chill / freeze


Kidney
____ 20 g, chill / freeze

Serum
____ 2 ml, seperated

Stomach Contents
____ 100 g, Chill / freeze

Feed or Source
____ 100 g, chill as required

Water
____ 100 ml, chill
C, U Cholinesterase Blood
___ 2 ml, EDTA tube, Chill

Brain
____ Whole or 1/2 (longitudinal)

 

STAT analysis is available, call lab.

C, U

Cyanide

Container note:
Paper Wrap Plants

Feed_ 200 g min, chill as required
Muscle
____ 20 g, chill

STAT analysis is available, call lab. Qualitative forage analysis

C, U
Endophyte fescue stem C
Floride Feed
____ 100 g

Water
____ 100 ml, chill

Bone


Urine
U Ethylene Glycol Liver
____ 20 g, chill

Kidney, whole
____chill / freeze
Blood
____ 3 ml, EDTA tube,chill
Serum
____ 5 ml, separated, freeze
Urine
____ 10 ml, chill
Source Material
____ 100 g, chill as required
C, U
Iron

Liver
____ 20 g, chill / freeze


Kidney
____ 20 g, chill / freeze

Serum
____ 2 ml, seperated

Stomach Contents
____ 100 g, chill / freeze

Feed or Source
____ 100 g, chilled as required

Water
____ 100 g, chill

C, U

Feed Quality

Feeds and Grains
___ 2 lb. mixed feed

___ representative sample


Hay and silage
____ 10 lb. corn
____ core samples
C
GC / MS Screen Contact Laboratory C, U
Ionophore Screen Feed
___ 100 g, chill as required

Screen for monensin, narasin, lasalocid, or salinomycin.

C, U
Lead

Liver
____ 20 g, chill / freeze


Kidney
____ 20 g, chill / freeze

Blood
____ 1ml, EDTA tube, chill

Stomach Contents
____ 100 g, chill / freeze

Feed or Source
____ 100 g, chilled as required

Water
____ 100 g, chill
C, U Metaldehyde Feed
____ 100 g, chill as required

Stomach Content
____ 100 g, freeze
Serum
____ 2 ml, freeze
C, U
Mycotoxin Screen

Feed, grain, milk, liver
____ 500 g, freeze


Indicate suspect toxin. Screen can include aflatoxins, ochratoxin-A, zearalenone, T-2 toxin, deoxynivalenol, and fumonisin, as well as others by special request.
C, U
Magnesium Serum
____ 2 ml, separated, chill

Feed
____ 100 g

Water
____ 100 ml, chill
C, U Nicotine Tissue
____ 100 g, chill

Stomach Contents
____ 100 g, chill
Urine
____ 20 ml, chill
C, U
Mercury Liver
____ 20 g, chill / freeze

Kidney
____ 20 g, chill / freeze

Serum
____ 2 ml, separated

Stomach Contents
____ 100 g, chill / freeze

Feed or Source
____ 100 g, chill as required

Water
___ 100 ml, chill
C, U Nitrate

Serum – Plasma

____ 2 ml, separated,

____ freeze or chill


Ocular Fluid
____ 1 ml, chill / freeze
Rumen, Abmasal Fluid
____ 5 ml, freeze or chill
Feed
____100 g, chill as required
Water
100 ml, chill
C, U
Molybdenum Liver
____ 20 g, chill / freeze

Kidney
____ 20 g, chill / freeze

Serum or Plasma
____ 2 ml, separated

Stomach Contents
____ 100 g, chill / freeze

Feed or Source
____ 100 g, chill as required

Water
___ 100 ml, chill
C, U Oxalate Kidney, whole
____ chill
Kidney, cross section
____ Formalin Fixation
Serum
____ 2 ml, chill/freeze
Urine
____ 2 ml, chill / freeze
C, U
Nickel Liver
____ 20 g, chill / freeze

Kidney
____ 20 g, chill / freeze

Serum or Plasma
____ 2 ml, separated

Stomach Contents
____ 100 g, chill / freeze

Feed or Source
____ 100 g, chill as required

Water
___ 100 ml, chill
C, U pH Serum
____ 2 ml, chill / freeze
Urine
____ 2 ml, chill / freeze
Rumen / Stomach Content
____ 100 g, chill / freeze
Water
____ 100 ml, chill
C, U
Nutrition Screen Serum
____ 4 ml, chill

U

Potassium

Serum
____


Ocular Fluid
____

Stomach Contents
____

Feed or Source
____

Water
____ 100 ml, chill
C, U Plant Identification

Wrap whole plant in paper, chill do not freeze.

 

Include root stock, stems, leaves, seeds, and fruits.

C, U
Rodenticide (Anticoagulant) Screen Liver____ 50 g, freeze
Kidney____ 50 g, freeze
Blood____ 10 ml, chill
Stomach Contents_____ 100 g, freeze
Feed____ 100 g, chill as required

Warfarin, coumafuryl, coumachlor, bromodiolone, brodifacoum, dicumarol, pindone, valone, diphacinone, and chlorophacinone can be screened. Time since exposure should be included in history.

C
Selenium Liver
____ 20 g, chill / freeze

Kidney
____ 20 g, chill / freeze

Whole Blood
____ 2 ml, (EDTA0, chill

Stomach Contents
____ 100 g, chill / freeze

Feed or Source
____ 100 g, chill / freeze

Water
____ 100 ml, chill
C, U Strychnine Liver
____ 50 g, freeze
Kidney
____ 50 g, freeze
Blood
____ 10 ml, chill
Urine
____ 10 ml, chill
Stomach Contents
_____ 100 g, freeze
Feed
____ 100 g, chill as required
Suspect Material
____ 100 g, freeze or chill
C, U

Sodium

(Toxic)/Salt Screen-Na

Brain
____ 20 g, chill

CSF
____ 2 ml, chill

Serum
____ 2 ml, separated, chill

Feed
____ 100 g, chill as required

Water
____ 100 ml, chill
C, U Urea Feed
____ 100 g, chill as required
Rumen / Stomach Contents
____ 100 g, freeze
C, U
Zinc phosphide Stomach Contents
____ 100 g, freeze or chill
Suspect Material
_____ 100 g, freeze or chill
C, U
Special Analyses by Request Special analyses can be configyred to accomplish client goals. Please consult with the diagnostic toxicologist.  
Thallium (See Arsenic) C, U      
Zinc

Note about Container:
Use trace element tube (royal blue top) for collection and shipment of serum without the clot (do not use red top clot tube.)


Liver
____ 20 g, chill / freeze
Kidney
____ 20 g, chill / freeze
Serum
____ 2 ml separated
Stomach Contents
____ 100 g, chill / freeze
Feed or Source
____ 100 g, chill as required
Water
____ 100 ml, chill
C, U      

Elemental Analysis Organic Analysis
General Toxicology Analysis Special Custom Analysis