xIllinois Veterinary Diagnostic Laboratories - Laboratory User's Manual
Appendix A — Specimen Selection and Submission —Infectious and Metabolic Diseases
| * C= Centralia | G=Galesburg | S=State Lab | U=University of Illinois |
| Disease |
Specimen
|
Discipline
|
Labs | Disease | Specimen | Discipline | Labs | |
|
Abortion |
--
|
--
|
--
|
Feline Infectious Peritonitis |
lung, liver, or organs w/ gross lesions fixed brain, eye, intestine, lung, liver, kidney, spleen serum, effusion fluids |
Virology
Histology Serology |
C,
G, U C, U C, U |
|
|
Actinomycosis/ |
abscess material fixed abscess lesion |
Bacteriology Histology |
C, G, U C, G, U |
Feline
Immunodeficiency Virus |
serum | Serology | C, U | |
|
Actinobacillus |
fresh
lung
fixed lung |
Bacteriology
Histology |
C, G,
U
C, G, U |
Feline Leukemia Virus | serum | Serology | C, U | |
| Adenovirus
infectious hepatitis (ICH, CAVI) |
fixed liver, kidney fresh nasal and throat swabs, cerebral spinal fluid |
Histology Virology
|
C, G, U C |
Feline Leukemia FOCMA | serum | Serology | C, U | |
| Laryngotracheitis
(CAV2), canine |
fixed
lung, trachea, liver, kidney
|
Histology
|
C, G, U | Glasser's Disease | See porcine polyserositis | ------- | ---- | |
| Aleutian Disease |
fixed lymph node, spleen,liver, kidney serum |
Histology Serology |
C, G, U C |
Heartworm (canine, feline Dirofilaria immitis infection) | 1 ml serum or plasma | Serology | C, U | |
| Anthrax |
blood,
spleen
|
Bacteriology
|
C, G, U | Hemagglutinating Encephalomyelitis Virus | fresh
brain, tonsil, mandibular lymph node, lung, kidney, spleen fixed brain |
Virology Histology
|
U C, G, U |
|
| Atrophic rhinitis |
nasal swabs 6-8 week old pigs delivered to laboratory same day fixed nasal turbinate |
Bacteriology Histology |
C, G, U C, G, U |
Hemophilus somnus
______Pneumonia
______TEM |
fresh lung fresh lung fresh brain fixed brain |
Bacteriology Histology Bacteriology Histology |
C,
G, U C, G, U C, G, U C, G, U |
|
|
Black Leg |
fresh skeletal muscle fixed skeletal muscle |
Bacteriology Histology |
C, G, U C, G, U |
Hemorrhagic
Bowel Syndrome |
fresh
affected small intestine, spiral colon fixed affected small intestine and spiral colon |
Bacteriology Histology |
C,
G, U C, G, U |
|
| Blood culture |
inoculated
blood culture system
|
Bacteriology
|
C, G, U | Inclusion Body Rhinitis | fixed nasal turbinate | Histology | C, G, U | |
| Bluetongue |
10 ml heparinized blood (Green top tubes) Serum (CF,SN,ELISA) |
Virology Serology |
C C, U |
Infectious Bovine
_________Abortion |
|
-------- |
----- |
|
| Border
Disease (BD/BVD) |
Dam:rectal, nasal swabs, whole blood (heparinized preferred), serum Lamb: 1/2 fresh brain, spinal cord, CSF,serum 1/2 fixed brain, spinal cord |
Virology Virology Histology |
C, G C, G, U C, G, U |
Johne's Disease | fresh
feces, ileum, spiral colon, regional mesenteric lymph node fixed ileum, spiral colon, regionalmesenteric lymph node serum milk |
Bacteriology Histology Serology Serology |
C,
G, U C, G, U C C |
|
| Borreliosis (canine Borrelia burgdorferi infection) |
1
ml serum
|
Serology
|
U | Laryngotracheitis (Herpes) Chicken |
trachea | Histology | U, G | |
| Botulism |
Serum
|
Bacteriology
|
G |
Leptospirosis _______Abortion _______Neurologic |
see
Abortion fresh kidney (not frozen) fixed kidney |
------
Bacteriology Histology |
----
C, G, U C, G, U |
|
| Bovine leukosis |
fixed lymph node and other involved organs serum |
Histology Serology |
G C, U |
Listeriosis
________Abortion ________Neurologic |
See
Abortion fresh 1/2 brain fixed 1/2 brain |
------- Bacteriology Histology |
C,
G, U C, G, U C, G, U |
|
| Bovine Mammillitis |
fresh vesicular fluid, scrapings, biopsy of lesion, serum fixed biopsy of lesion |
Virology
Histology |
U
C, G, U |
Lyme disease |
1 ml serum | Serology | U | |
| Bovine
Respiratory Syncytial |
fresh lung, tracheal lavage fluid Virus fixed lung |
Virology Histology |
C, G, U C, G, U |
Malignant
Catarrhal Fever |
heparinized
blood fixed brain, lung, liver kidney, spleen, lymph node serum |
Virology Histology Virology Serology |
C, G, U C, G, U |
|
| Bovine
Virus Diarrhea |
fresh abomasum, ileum, spleen, lymph node; fetus (kidney, liver, lung, spleen) fixed abomasum, ileum serum(chronic cases) |
Histology
Virology Virology |
C, G, U
C, U C, U |
Mastitis | fresh
milk; use proper sample container half full or less. Call Lab before submitting large (>15) numbers of samples |
Bacteriology Bacteriology |
C,
G, U C, G, U |
|
| Bovine
Virus Diarrhea screening test |
serum |
Virology
|
U | Mycoplasma Arthritis | fresh
joint fluid or swab fixed synovial membrane |
Bacteriology Histology |
C,
G, U C, G, U |
|
|
Brucellosis ________ Livestock ________ Canine |
-----
Bacteriology Serology |
C, G,
U
C, G, U C, G, U |
Mycoplasma hyorhinis | See porcine polyserositis |
-----
|
----
|
||
|
Campylobacter/ |
-----
|
C, G, U | Mycoplasma pneumonia |
fresh
lung fixed lung |
Bacteriology Histology |
C,
G, U C, G, U |
||
| Canine Distemper |
fresh
urinary bladder, lung, brain, conjunctival smear, heparanized blood
fixed brain, lung, urinary bladder, stomach, liver, kidney |
Virology Histology
|
C, G, U
C, G, U
|
Nocardiosis | fresh
involved organ or lesion fixed lesion |
Bacteriology Histology |
C,
G, U C, G, U |
|
| Canine Herpesvirus |
fixed
lung, liver, kidney, brain
fixed lung, liver, kidney, brain |
Virology
Histology |
C, G, U C, G, U
|
Ovine
Progressive Pneumonia (Visna, Maedi) |
fixed
lung serum |
Histology Serology |
C,
G, U C, U |
|
|
Canine Parvovirus/ |
fresh
small intestine (multiple sections), feces,
fixed small intestine, spleen, mesenteric lymph nodes |
Virology Histology
|
C, G, U C, G, U
|
Porcine Enterovirus | serum | Serology | C | |
| Caprine Contagious Arthritis/Encephalitis |
fixed
brain, synovial membrane, lung, mammary gland
serum |
Histology Serology
|
C, G, U C, U
|
Porcine Parvovirus | fresh
mummified fetus or fetal lung paired serum |
Virology Serology |
C,
G, U C, U |
|
| Caseous Lymphadenitis |
fresh
abscessed lymph
fixed abscessed lymph node |
Bacteriology
Histology |
C, G,
U C, G, U |
Porcine Polyserositis | fresh
meningeal, peritoneal, pleural and/or joint swabs,lung fixed brain, heart, lung, liver, spleen |
Bacteriology Histology |
C,
G, U C, G, U |
|
|
Chlamydia
______Avian ______Feline |
See
Abortion
fresh lung, liver, nasal swabs, feces fixed lung, liver, ocular fresh ocular,
* conjunctival scrapings |
Virology Histology Histology
|
C, G, U C, G, U
G C, U |
Porcine Reproductive
__Respiratory Syndrome |
fresh
lung, serum fixed brain, heart, lung serum |
Virology Histology Serology |
U,
G C, G, U C, G, U |
|
| Clostridium perfringens |
fresh
intestine, or intestinal contents
fixed duodenum, jejunum, ileum, colon |
Bacteriology
Histology |
C, G C, G, U |
Proliferative Ileitis | fixed ileum, spiral colon | Histology | C, G, U | |
| Coccidiosis |
feces,
intestine smears
fixed duodenum, jejunum, ileum, and colon |
Parasitology
Histology |
C, G,
U C, G, U |
Pseudo-cowpox | fresh
vesicular fluid, scrapings biopsy of lesion, serum fixed biopsy |
Virology Histology |
U C, G, U |
|
|
Colibacillosis ______Mammalian
______Avian |
fresh duodenum, jejunum and ileum fixed duodenum,
jejunum, ileum, colon fresh liver, air sac swab fixed liver, spleen, air sac
|
Bacteriology
Histology |
C, G, U
C, G ,U |
Pseudorabies | fresh
brain, lung, tonsils, spleen fixed brain serum |
Virology Histology Serology |
C,
G, U C,G,U C, G |
|
| Contagious
Ecthyma (sore mouth, ORF) |
fresh
skin scrapings and crusts, vesicular fluid, serum
fixed biopsy |
Virology
Histology |
C, U C, G, U |
Rabies | fresh brain | Virology | C, G | |
| Contagious Equine Metritis |
cervical
secretions or male urethral fossa secretions (call
laboratory for instructions)
|
Bacteriology
|
C | Rhodococcus equi | fresh lung, or other affected tissue | Bacteriology | C, G, U | |
|
Coronavirus/Rotavirus _______Calf diarrhea _______Porcine |
fresh
jejunum, ileum, spiral colon, intentinal contents or feces
(< 24 hours of onset) fixed duodenum, jejunum, ileum, spiral colon See Transmissible Gastroenteritis |
Virology
Histology |
C, G,
U C, G, U |
Rotavirus ________Calf ________ Porcine |
See
Corona Virus fresh jejunum, ileum, fecal fixed duodenum, jejunum, ileum, colon |
----
Virology Histology |
---
C, G, U C, G, U |
|
| Cryptosporidiosis |
fresh
intestine, fecal or intestinal smear, feces
fixed ileum, spiral colon |
Parasitology
Histology |
C, G C, G, U |
Salmonellosis | fresh
lung, liver, jejunum, ileum, mesenteric lymph node, colon, colonic lymph
node, kidney, spleen fixed lung, liver, duodenum,jejunum, ileum, colon, kidney, spleen, lymph nodes |
Bacteriology Histology |
C,
G, U C, G, U |
|
| Dermatophilus |
fresh
scabs, exudate
fixed skin |
Bacteriology
Histology |
C, G,
U C, G, U |
Salt Poisoning | fixed
brain serum or cerebral spinal fluid brain, 20 g, chill |
Histology Clin Path Toxicology |
C,
G, U C, G C, U |
|
| Dirofilariasis (canine, feline Dirofilaria immitis infection) |
1
ml serum or plasma
|
Serology
|
U | Semen
Testing, Bovine Viral Isolation |
6 straws or equivalent per animal frozen in liquid nitrogen | Virology | C | |
| Edema Disease |
fresh
jejunum and ileum
fixed brain, stomach, spiral colon |
Bacteriology
Histology |
C, G,
U C, G, U |
__Parainfluenza(PI-3) |
fresh
lung, trachea, ocular swabs, nasal swabs, respiratory lymph nodes, spleen
fixed lung, trachea paired serum fresh lung, trachea paired serum |
Bacteriology Histology Serolology Virology Serology |
C,
G, U C, G, U C, U U C, U |
|
| Epididymitis – Ovine |
semen
semen, serum |
Bacteriology
Serology |
C, G,
U G |
Strangles | abscess exudate | Bacteriology | C, G, U | |
| Erysipelas |
fresh
liver, kidney, spleen, lymph nodes
|
Bacteriology
|
C, G, U | Swine Dysentery | fresh
spiral colon fixed spiral colon |
Bacteriology Histology |
C,
G, U Histology |
|
| Equine Adenovirus |
live: nasal
and conjunctival swabs dead: lung, parotid salivary glands, pancreas, spleen, thymus, lymph nodes fixed lung |
Virology Histology |
U C, G, U |
Swine Influenza |
fresh lung, trachea, respiratory lymph nodes fixed lung paired serum |
Virology Histology Serology |
C,
G, U C, G, U U |
|
| Equine
Coital Exanthema(EHV-3) |
vesicular fluid of genital lesions | Virology | U | Systemic Fungus | fresh
involved organ fixed lung, liver, other involved organs serum |
Bacteriology Histology Serology |
C,
G, U C, G, U U |
|
| Equine Encephalomyelitis | Equine
Encephalomyelitis fixed brain, spinal cord paired serum |
Virology Histology Serology |
C, G, U |
Toxoplasmosis | serum | Serology | C | |
| Equine Infectious Anemia | fixed
heart, liver, kidney, spleen serum |
Histology Serology |
C,
G, U S, U |
Transmissible Gastroenteritis | fresh
jejunum and terminal ileum (do not freeze),
diarrheic feces fixed duodenum, jejunum, ileum, spiral colon paired serum |
Virology Histology Serology |
C,
G, U C, G, U C, G, U |
|
| Equine Infertility | uterine
swab uterine biopsy (best if obtained at estrus) |
Bacteriology Histology |
C,
G, U C, G, U |
Trichomoniasis | See
Abortion uterine exudate, preputial scraping |
Bacteriology | C, G | |
| Equine Influenza | nasal/pharyngeal swabs | Virology | C | Tuberculosis | fresh
involved tissue * fixed involved tissue (samples will be forwarded to NVSL) |
Bacteriology Histology |
G,
C C, G, U |
|
|
Equine
_______Abortion _______Neurologic |
fresh
lung and trachea, nasal/pharyngeal swabs, tracheal lavage fluid fixed lung paired serum __________________ See Abortion fresh lung, liver, spleen, kidney, thymus fixed liver, adrenal, lung, placenta fresh brain, spiral cord, cerebral spinal fluid fixed brain, spinal cord |
Virology Histology Serology _________ Virology Histology Virology Histology |
C,
G, U C, G, U C, U ____ C, G, U C, G, U C, G, U C, G, U |
Tyzzer's Disease | fixed liver, ileum, colon | Histo | C, G, U | |
| Equine
Viral Arteritis ____________Abortion |
fresh
lung, bronchial lymph nodes, kidney, spleen paired serum fresh kidney, lung, spleen fixed liver, lung, kidney, placenta, spleen |
Virology Serology Virology Histology |
U C, U U C, G, U |
Vesicular Stomatitis | fresh
vesicular fluid, scrapings, biopsy, serum fixed biopsy of lesion serum |
Virology Histology Serology |
*
, U C, G, U C |
|
| Feline Calicivirus | fresh
nasal, ocular, pharyngeal, oral ulcer swabs, lung, or bronchial lymph node,
spleen fixed lung, oral ulcers paired serum |
Virology Histology Serology |
C,
G, U C, G, U U |
Weak Calf Syndrome (BVDV) | fresh
lung, liver, spleen, lymph node fixed brain, heart, lung, liver, kidney, skeletal muscle serum |
Bacteriology Virology Histology Serology |
C,
G, U C, G, U C, U |
|
| Feline
Rhinotracheitis/ Herpesvirus |
fresh
nasal, ocular, pharyngeal, oral ulcer swabs, lung, bronchial lymph nodes,
spleen fixed lung, oral ulcers paired serum |
Virology Histology Serology |
C,
G, U C, G, U U |
White Muscle Disease | fixed
skeletal muscle, heart, tongue serum, liver |
Histology Chemistry |
C,
G, U C, U |
|
| Feline
Panleukopenia/ Parvovirus |
fresh
duodenum, jejunum, ileum fixed jduodenum, jejunum, ileum paired serum |
Virology
Histology Serology |
C,
G, U C, G, U U |
White Muscle Disease | fixed
skeletal muscle, heart, tongue serum, liver |
Histology Chemistry |
C,
G, U C, U |
|
|
* Sent to Reference Laboratory; contact for details
Revised 1-1-00 website designer Lou Ann Miller |
||||||||