Illinois Department of Agriculture - Animal Disease Laboratories
Centralia - Galesburg - Springfield - University of Illinois, VDL
Laboratory User's Manual
 
User Manual


Fees
Contacts
Submissions
Necropsy
Histopathology
Bacteriology
Mycology
Virology
Serology
Immunology
Toxicology
Toxicology Chemistry
Parasitology
Endocrinology
Clinical Pathology

Rabies
Reportable Diseases
Consideration

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(Revised 3-11-01) Web Designer: Lou Ann Miller

Appendix A: Infectious & Metabolic Diseases

 


* C= Centralia G=Galesburg S=State Lab U=University of Illinois

Disease
Specimen
Discipline
Labs                                   Disease Specimen Discipline Labs

Abortion

(See User manual)

--
--
--
Feline Infectious Peritonitis lung, liver, or organs w/ gross lesions

fixed brain, eye, intestine, lung, liver, kidney, spleen

serum, effusion fluids
Virology


Histology


Serology
C, G, U


C, U


C, U

Actinomycosis/
Actinobacillosis

abscess material


fixed abscess lesion

Bacteriology



Histology

C, G, U



C, G, U
Feline
Immunodeficiency Virus
serum Serology C, U

Actinobacillus
pleuropneumoniae

fresh lung
fixed lung
Bacteriology
Histology
C, G, U
C, G, U
Feline Leukemia Virus serum Serology C, U
Adenovirus
infectious hepatitis
(ICH, CAVI)

fixed liver, kidney


fresh nasal and throat swabs, cerebral spinal fluid

Histology


Virology

 

C, G, U


C
Feline Leukemia FOCMA serum Serology C, U
Laryngotracheitis
(CAV2)
, canine
fixed lung, trachea, liver, kidney
Histology
C, G, U Glasser's Disease See porcine polyserositis ------- ----
Aleutian Disease

fixed lymph node, spleen,liver, kidney


serum

Histology



Serology

C, G, U


C
Heartworm (canine, feline Dirofilaria immitis infection) 1 ml serum or plasma Serology C, U
Anthrax
blood, spleen
Bacteriology
C, G, U Hemagglutinating Encephalomyelitis Virus fresh brain, tonsil, mandibular lymph node, lung, kidney, spleen

fixed brain

Virology


Histology

 

U



C, G, U
Atrophic rhinitis

nasal swabs


6-8 week old pigs delivered to laboratory same day fixed nasal turbinate

Bacteriology


Histology

C, G, U


C, G, U

Hemophilus somnus

 

______Pneumonia

 

______TEM

fresh lung


fresh lung

fresh brain

fixed brain
Bacteriology


Histology

Bacteriology

Histology
C, G, U


C, G, U

C, G, U

C, G, U

Black Leg
/Malignant Edema

fresh skeletal muscle


fixed skeletal muscle

Bacteriology


Histology

C, G, U


C, G, U
Hemorrhagic
Bowel Syndrome
fresh affected small intestine, spiral colon

fixed affected small intestine and spiral colon
Bacteriology


Histology
C, G, U


C, G, U
Blood culture
inoculated blood culture system
Bacteriology
C, G, U Inclusion Body Rhinitis fixed nasal turbinate Histology C, G, U
Bluetongue

10 ml heparinized blood (Green top tubes)


Serum (CF,SN,ELISA)

Virology


Serology

C



C, U

Infectious Bovine
Rhinotracheitis


_________Respiratory

_________Abortion

 


see Shipping Fever

see Abortion

 

 

--------

 

 

-----

Border Disease
(BD/BVD)

Dam:rectal, nasal swabs, whole blood (heparinized preferred), serum


Lamb: 1/2 fresh brain, spinal cord, CSF,serum


1/2 fixed brain, spinal cord

Virology



Virology



Histology

C, G



C, G, U



C, G, U
Johne's Disease fresh feces, ileum, spiral colon, regional mesenteric lymph node

fixed ileum, spiral colon, regionalmesenteric lymph node

serum

milk
Bacteriology



Histology



Serology

Serology

C, G, U



C, G, U



C

C
Borreliosis
(canine Borrelia burgdorferi infection)
1 ml serum
Serology
U Laryngotracheitis
(Herpes) Chicken
trachea Histology U, G
Botulism
Serum
Bacteriology
G

Leptospirosis

_______Abortion

_______Neurologic

see Abortion

fresh kidney (not frozen)

fixed kidney
------

Bacteriology

Histology
----

C, G, U

C, G, U
Bovine leukosis

fixed lymph node and other involved organs


serum

Histology



Serology

G
C, U


C, U
Listeriosis

________Abortion

________Neurologic

See Abortion

fresh 1/2 brain

fixed 1/2 brain
-------

Bacteriology

Histology
C, G, U

C, G, U

C, G, U
Bovine Mammillitis

fresh vesicular fluid, scrapings, biopsy of lesion, serum


fixed biopsy of lesion

Virology

 


Histology

U

 


C, G, U

Lyme disease
(canine Borrelia
burgdorferi infection)

1 ml serum Serology U
Bovine Respiratory
Syncytial

fresh lung, tracheal lavage fluid


Virus fixed lung

Virology



Histology

C, G, U



C, G, U
Malignant
Catarrhal Fever
heparinized blood

fixed brain, lung, liver

kidney, spleen, lymph node

serum
Virology

Histology

Virology


Serology
*

C, G, U

C, G, U


Bovine Virus
Diarrhea

fresh abomasum, ileum, spleen, lymph node; fetus (kidney, liver, lung, spleen)


fixed abomasum, ileum
serum(chronic cases)

Histology




Virology
Virology

C, G, U




C, U
C, U
Mastitis fresh milk; use proper sample container half full or less.

Call Lab before submitting large (>15) numbers of samples
Bacteriology



Bacteriology
C, G, U



C, G, U
Bovine Virus
Diarrhea screening test

serum
(animals > 2 mon. age)

Virology
U Mycoplasma Arthritis fresh joint fluid or swab

fixed synovial membrane
Bacteriology

Histology
C, G, U

C, G, U

Brucellosis

________ Livestock

________ Canine

See Abortion
heparinized blood
Serum
-----

Bacteriology
Serology
C, G, U
C, G, U
C, G, U
Mycoplasma hyorhinis See porcine polyserositis
-----
----

Campylobacter/
abortion

-----
C, G, U Mycoplasma
pneumonia
fresh lung

fixed lung
Bacteriology

Histology
C, G, U

C, G, U
Canine Distemper
fresh urinary bladder, lung, brain, conjunctival smear, heparanized blood
fixed brain, lung, urinary bladder, stomach, liver, kidney

Virology



Histology

 

C, G, U

 


C, G, U

 

Nocardiosis fresh involved organ or lesion

fixed lesion
Bacteriology


Histology
C, G, U


C, G, U
Canine Herpesvirus
fixed lung, liver, kidney, brain
fixed lung, liver, kidney, brain
Virology


Histology

C, G, U


C, G, U

 

Ovine Progressive
Pneumonia
(Visna, Maedi)
fixed lung

serum
Histology

Serology
C, G, U

C, U

Canine Parvovirus/
Coronavirus

fresh small intestine (multiple sections), feces,
fixed small intestine, spleen, mesenteric lymph nodes

Virology


Histology

 

C, G, U


C, G, U

 

Porcine Enterovirus serum Serology C
Caprine Contagious Arthritis/Encephalitis
fixed brain, synovial membrane, lung, mammary gland

serum

Histology


Serology

 

C, G, U



C, U

 

Porcine Parvovirus fresh mummified fetus or fetal lung

paired serum
Virology


Serology
C, G, U


C, U
Caseous Lymphadenitis
fresh abscessed lymph

fixed abscessed lymph node
Bacteriology

Histology
C, G, U

C, G, U
Porcine Polyserositis fresh meningeal, peritoneal, pleural and/or joint swabs,lung

fixed brain, heart, lung, liver, spleen
Bacteriology



Histology
C, G, U



C, G, U

Chlamydia


______Abortion


______Avian
______Feline
See Abortion


fresh lung, liver, nasal swabs, feces


fixed lung, liver, ocular

fresh ocular,
pharyngeal swabs


* fixed conjunctiva, lung

* conjunctival scrapings




Virology
Histology



Histology


Histology


Virology




C, G, U



C, G, U

 

G

C, U

Porcine Reproductive

 

__Respiratory Syndrome

fresh lung, serum

fixed brain, heart, lung

serum
Virology

Histology

Serology
U, G

C, G, U

C, G, U
Clostridium perfringens
fresh intestine, or intestinal contents

fixed duodenum, jejunum, ileum, colon
Bacteriology


Histology
C, G


C, G, U
Proliferative Ileitis fixed ileum, spiral colon Histology C, G, U
Coccidiosis
feces, intestine smears

fixed duodenum, jejunum, ileum, and colon
Parasitology

Histology
C, G, U

C, G, U
Pseudo-cowpox fresh vesicular fluid, scrapings biopsy of lesion, serum

fixed biopsy
Virology



Histology

U


C, G, U

Colibacillosis

______Mammalian

 

 

 

______Avian

 

fresh duodenum, jejunum and ileum

fixed duodenum, jejunum, ileum, colon



fresh liver, air sac swab

fixed liver, spleen, air sac

 

 

Bacteriology

 

 


Histology

 

C, G, U

 

 



C, G ,U
Pseudorabies fresh brain, lung, tonsils, spleen

fixed brain

serum
Virology


Histology

Serology
C, G, U


C,G,U

C, G
Contagious Ecthyma
(sore mouth, ORF)
fresh skin scrapings and crusts, vesicular fluid, serum

fixed biopsy
Virology



Histology
C, U



C, G, U
Rabies fresh brain Virology C, G
Contagious
Equine Metritis
cervical secretions or male urethral fossa secretions (call laboratory for instructions)
Bacteriology
C Rhodococcus equi fresh lung, or other affected tissue Bacteriology C, G, U

Coronavirus/Rotavirus

_______Calf diarrhea

_______Porcine

fresh jejunum, ileum, spiral colon, intentinal contents or feces
(< 24 hours of onset)


fixed duodenum, jejunum, ileum, spiral colon

See Transmissible Gastroenteritis
Virology





Histology


C, G, U





C, G, U


Rotavirus

________Calf

________ Porcine

See Corona Virus

fresh jejunum, ileum, fecal

fixed duodenum, jejunum, ileum, colon
----

Virology

Histology
---

C, G, U

C, G, U
Cryptosporidiosis
fresh intestine, fecal or intestinal smear, feces

fixed ileum, spiral colon
Parasitology



Histology

C, G


C, G, U
                  Salmonellosis fresh lung, liver, jejunum, ileum, mesenteric lymph node, colon, colonic lymph node, kidney, spleen

fixed lung, liver, duodenum,jejunum, ileum, colon, kidney, spleen, lymph nodes
Bacteriology





Histology



C, G, U





C, G, U
Dermatophilus
fresh scabs, exudate

fixed skin
Bacteriology

Histology
C, G, U

C, G, U
Salt Poisoning fixed brain

serum or cerebral spinal fluid

brain, 20 g, chill
Histology

Clin Path


Toxicology
C, G, U


C, G

C, U
Dirofilariasis
(canine, feline Dirofilaria
immitis infection)
1 ml serum or plasma
Serology
U Semen Testing,
Bovine Viral Isolation
6 straws or equivalent per animal frozen in liquid nitrogen Virology C
Edema Disease
fresh jejunum and ileum

fixed brain, stomach, spiral colon
Bacteriology


Histology
C, G, U


C, G, U

Shipping Fever (Bovine)

 

__Parainfluenza(PI-3)

fresh lung, trachea, ocular swabs, nasal swabs, respiratory lymph nodes, spleen

fixed lung, trachea

paired serum
fresh lung, trachea
paired serum
Bacteriology




Histology

Serolology

Virology

Serology
C, G, U




C, G, U

C, U

U

C, U
Epididymitis – Ovine
semen

semen, serum
Bacteriology

Serology
C, G, U

G
Strangles abscess exudate Bacteriology C, G, U
Erysipelas
fresh liver, kidney, spleen, lymph nodes
Bacteriology
C, G, U Swine Dysentery fresh spiral colon

fixed spiral colon
Bacteriology

Histology
C, G, U

Histology
Equine Adenovirus

live: nasal and conjunctival swabs


dead: lung, parotid salivary glands, pancreas, spleen, thymus, lymph nodes fixed lung
Virology



Histology


U



C, G, U
Swine Influenza fresh lung, trachea, respiratory lymph nodes

fixed lung

paired serum
Virology


Histology

Serology

C, G, U


C, G, U

U
Equine Coital
Exanthema
(EHV-3)
vesicular fluid of genital lesions Virology U Systemic Fungus fresh involved organ

fixed lung, liver, other involved organs

serum
Bacteriology

Histology


Serology
C, G, U

C, G, U


U
Equine Encephalomyelitis Equine Encephalomyelitis

fixed brain, spinal cord

paired serum
Virology


Histology

Serology
*


C, G, U

Toxoplasmosis serum Serology C
Equine Infectious Anemia fixed heart, liver, kidney, spleen

serum
Histology


Serology
C, G, U


S, U
Transmissible Gastroenteritis fresh jejunum and terminal ileum (do not freeze), diarrheic feces

fixed duodenum, jejunum, ileum, spiral colon

paired serum
Virology



Histology


Serology
C, G, U



C, G, U


C, G, U
Equine Infertility uterine swab

uterine biopsy (best if obtained at estrus)
Bacteriology

Histology
C, G, U

C, G, U
Trichomoniasis See Abortion

uterine exudate, preputial scraping
Bacteriology C, G
Equine Influenza nasal/pharyngeal swabs Virology C Tuberculosis fresh involved tissue *

fixed involved tissue (samples will be forwarded to NVSL)
Bacteriology

Histology
G, C

C, G, U

Equine
Rhinopneumonitis

 

 

_______Abortion


===================


_______Neurologic


fresh lung and trachea, nasal/pharyngeal swabs, tracheal lavage fluid

fixed lung

paired serum

__________________
See Abortion
fresh lung, liver, spleen, kidney, thymus

fixed liver, adrenal, lung, placenta

fresh brain, spiral cord, cerebral spinal fluid

fixed brain, spinal cord
Virology




Histology

Serology

_________
Virology




Histology


Virology


Histology
C, G, U




C, G, U

C, U

____
C, G, U




C, G, U


C, G, U


C, G, U
Tyzzer's Disease fixed liver, ileum, colon Histo C, G, U
Equine Viral Arteritis





____________Abortion
fresh lung, bronchial lymph

nodes, kidney, spleen paired serum

fresh kidney, lung, spleen

fixed liver, lung, kidney, placenta, spleen
Virology


Serology


Virology


Histology
U


C, U


U


C, G, U
Vesicular Stomatitis fresh vesicular fluid, scrapings, biopsy, serum

fixed biopsy of lesion

serum
Virology


Histology

Serology
* , U


C, G, U

C
Feline Calicivirus fresh nasal, ocular, pharyngeal, oral ulcer swabs, lung, or bronchial lymph node, spleen

fixed lung, oral ulcers

paired serum
Virology




Histology

Serology
C, G, U




C, G, U

U
Weak Calf Syndrome (BVDV) fresh lung, liver, spleen, lymph node

fixed brain, heart, lung, liver, kidney, skeletal muscle

serum
Bacteriology
Virology

Histology



Serology
C, G, U


C, G, U



C, U
Feline Rhinotracheitis/
Herpesvirus
fresh nasal, ocular, pharyngeal, oral ulcer swabs, lung, bronchial lymph nodes, spleen

fixed lung, oral ulcers

paired serum
Virology




Histology

Serology
C, G, U




C, G, U

U
White Muscle Disease fixed skeletal muscle, heart, tongue

serum, liver
Histology


Chemistry
C, G, U


C, U
Feline Panleukopenia/
Parvovirus
fresh duodenum, jejunum, ileum

fixed jduodenum, jejunum, ileum

paired serum
Virology


Histology


Serology
C, G, U


C, G, U


U
White Muscle Disease fixed skeletal muscle, heart, tongue

serum, liver
Histology


Chemistry
C, G, U


C, U

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


* Sent to Reference Laboratory; contact for details