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Graduate Student Expo 2011

Mingyuan Han, PhD candidate
Structure function relationship of non-structural protein of porcine reproductive and respiratory syndrome virus
Advisor:  D. Yoo

Porcine reproductive and respiratory syndrome (PRRS) continues to be a threat to the swine industry worldwide. The etiological agent of this disease, porcine reproductive and respiratory syndrome virus (PRRSV), belongs to the family Arteriviridae and possesses a single-stranded positive-sensed RNA genome. Both structural proteins and nonstructural proteins are essential for PRRSV. PRRSV nonstructural proteins are produced by proteolytic processing after generation of polypeptide 1a (pp1a) and polypeptide 1ab (pp1ab). Downregualtion of interferon-β production by PRRSV had been identified. Nsp1 is one of the viral proteins were proved that can cause IFN-β downregulation, and CREB-binding protein (CBP), an important element for IFN modulation, was degraded in the presence of Nsp1. Previous study has shown that Nsp1α inhibited NF-κB activation and thus suppressed the production of IFN-β. Containing motifs like zinc finger, protease and nuclease, the function structure relationship between IFN-β downregulation and these motifs of Nsp1α and Nsp1β is under investigation currently.