Transgenic Animals 2017-11-10T22:43:37+00:00

Transgenic animals

We created the following transgenic mice and made them available to the research community. These transgenic mouse lines are being transferred to the Jackson Laboratory for a wider distribution.

 

Cyp17-iCre.  This is the first and most widely used transgenic mouse line among those created by my laboratory.  This mouse is used for conditional deletion of genes in the androgen-producing cells and also for localizing cells that synthesize androgens.  The generation and characterization of this line of mice was published in Genesis in 2008.
Reference: Generation of Cyp17iCre transgenic mice and their application to conditionally delete estrogen receptor alpha (Esr1) from the ovary and testis. Bridges PJ, Koo Y, Kang DW, Hudgins-Spivey S, Lan ZJ, Xu X, DeMayo F, Cooney A, Ko C. Genesis. 2008 Sep;46(9):499-505. doi: 10.1002/dvg.20428.
Jax Mice: https://www.jax.org/strain/028547

 

Esr2-iCre. This mouse line is used for conditional deletion of genes in the ERβ (estrogen receptor beta)-expressing cells.  This line is also used for localizing the sites of ERβ expression.  The generation of this line was published in Genesis in 2016.  A grant was submitted to NIH for the use of this model in studying estrogen receptors in the ovary.
Reference: Generation of an estrogen receptor beta-iCre knock-in mouse. Cacioppo JA, Koo Y, Lin PC, Osmulski SA, Ko CD, Ko C. Genesis. 2016 Jan;54(1):38-52. doi: 10.1002/dvg.22911.
JaxMice: https://www.jax.org/strain/028717

 

Esr1-iCre. This mouse line is used for conditional deletion of genes in the ERα (estrogen receptor alpha)-expressing cells.  This line is also used for localizing the sites of ERα expression.  The generation of this line is yet to be reported as a manuscript.
Reference: Generation and characterization of an estrogen receptor alpha-iCre knock-in mouse. Park CJ, Chen G, Koo Y, Lin PP, Cacioppo JA, Prohaska H, Ko CJ. Genesis. 2017 Nov 8. doi: 10.1002/dvg.23084. [Epub ahead of print]

 

End2-iCre.  This mouse line is used for conditional deletion of genes in endothelin-2 expressing cells and for localizing the sites of endothelin-2 expression.  The generation of this line of mice was published in February 2015 as a cover image of the journal Genesis.  There is a high demand of this line of mice from a variety of disciplines of science.
Reference: Generation and characterization of an endothelin-2 iCre mouse. Cacioppo JA, Koo Y, Lin PC, Gal A, Ko C. Genesis. 2015 Feb;53(2):245-56. doi: 10.1002/dvg.22845.

 

Edn2-flox. This mouse line is used for conditional deletion of endothelin-2 gene.  We used this line of mice for demonstrating the critical role that endothelin-2 plays in ovulation (Scientific Reports, 2017).  Endothelin-2 is also expressed in the eyes, intestines and kidneys.  This line of mice is already being requested by other laboratories.
Reference: Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture. Cacioppo JA, Lin PP, Hannon PR, McDougle DR, Gal A, Ko C. Sci Rep. 2017 Apr 11;7(1):817. doi: 10.1038/s41598-017-00943-w.