The long-term objective of our laboratory research is to elucidate the molecular mechanism of ovulation, the process of egg release from the ovary. The program of ovulation is activated by a surge of pituitary luteinizing hormone that initiates dramatic changes in molecular, biochemical, and physical events in the ovary, eventually expelling eggs from the ovary. For normal ovulation, the function of various cells including the granulosa and theca cell constituting the ovary is important. Functional abnormalities of the genes expressed in these cells may cause ovulatory failure. The current study in our laboratory focuses on the function of the hormone receptors expressed in the ovary and its interactions with downstream events. We have generated transgenic animal models and used them for those projects. Once noble findings are made from the animal studies, molecular mechanisms behind are sought using in vitro experiments. Routinely applied skills/approaches include but not limited to tissue specific gene targeting, microsurgery, organ transplantation, fertility test, histology, immunohistochemistry, hormone assays, DNA microarray, real-time PCR, tissue culture, and bioinformatics. The ultimate research goal of our lab is to help women who suffer from reproductive disorders and to develop safe and effective contraceptive strategies for humans and animals. In addition, we would like to suggest ways to improve various diseases caused by functional abnormalities of steroid hormone receptors.