Canine Influenza in McLean County (Illinois)

May 16, 2016 / Diagnostic Updates

Canine Influenza

Recently we have tested many samples for canine influenza virus from the Bloomington/Normal area with many of them testing positive. A small number of samples have been forwarded for subtyping and they have come back H3N2. Below is information regarding sample submission and testing guidelines. As always if you have any questions please feel free to contact the lab.

Influenza A rRT-PCR: Specimens must be received by 4:00 PM Mon. or Wed. for next business day testing.
Swabs should be moist but not in transport media or PBS. If necessary, use 1 drop of sterile water in a sterile tube.
If possible use nylon swabs with plastic sticks instead of cotton with wooden sticks (cotton & wood inhibit the PCR).
Use aseptic techniques to avoid cross-contamination.
Please label specimens clearly and indicate what type of specimen is being submitted.
Serum is not an appropriate specimen.

Porcine: IAV (SIV) rRT-PCR –
2 nasopharyngeal swabs (one from each nostril or each side of the pharynx), oral fluid or fresh/frozen lung.
Please seal specimen tubes well and do not overfill them as this can cause contamination of other specimens.
Subtyping assay for H1/H3 and N1/N2 is available.
Please include age of animal and date of specimen collection for surveillance testing through the USDA.
USDA/NAHLN offers SIV surveillance testing for up to 5 oral fluids and up to 10 nasal swabs or lung tissue.
Of positive specimens with Ct value <30 for oral fluids and <35 for nasal swabs or lung, only 2 will be subtyped.
Of the 2 specimens that are subtyped, only one successful virus isolation may be sent out for sequencing.
Sequencing may take a month as the facility that does the sequencing is receiving many samples everyday.

Avian: IAV (AIV) rRT-PCR –
choanal/cloacal swab, tracheal swabs (one from each side of the trachea) or fresh/ frozen lung.
Subtyping assay for H5 and H7 is available.

Canine: Influenza A Matrix rRT-PCR –
2 nasopharyngeal swabs (one from each nostril or each side of the pharynx) or fresh/ frozen lung.

Equine: Influenza A Matrix (EqInFV) rRT-PCR – (separate from the Equine Respiratory Panel)
2 nasopharyngeal swabs (one from each nostril or each side of the pharynx) or fresh/ frozen lung.